Increased fMRI connectivity upon chemogenetic inhibition of the mouse prefrontal cortex.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
25 02 2022
25 02 2022
Historique:
received:
04
01
2021
accepted:
27
01
2022
entrez:
26
2
2022
pubmed:
27
2
2022
medline:
13
4
2022
Statut:
epublish
Résumé
While shaped and constrained by axonal connections, fMRI-based functional connectivity reorganizes in response to varying interareal input or pathological perturbations. However, the causal contribution of regional brain activity to whole-brain fMRI network organization remains unclear. Here we combine neural manipulations, resting-state fMRI and in vivo electrophysiology to probe how inactivation of a cortical node causally affects brain-wide fMRI coupling in the mouse. We find that chronic inhibition of the medial prefrontal cortex (PFC) via overexpression of a potassium channel increases fMRI connectivity between the inhibited area and its direct thalamo-cortical targets. Acute chemogenetic inhibition of the PFC produces analogous patterns of fMRI overconnectivity. Using in vivo electrophysiology, we find that chemogenetic inhibition of the PFC enhances low frequency (0.1-4 Hz) oscillatory power via suppression of neural firing not phase-locked to slow rhythms, resulting in increased slow and δ band coherence between areas that exhibit fMRI overconnectivity. These results provide causal evidence that cortical inactivation can counterintuitively increase fMRI connectivity via enhanced, less-localized slow oscillatory processes.
Identifiants
pubmed: 35217677
doi: 10.1038/s41467-022-28591-3
pii: 10.1038/s41467-022-28591-3
pmc: PMC8881459
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1056Subventions
Organisme : NIMH NIH HHS
ID : R21 MH116473
Pays : United States
Organisme : NINDS NIH HHS
ID : U19 NS107464
Pays : United States
Informations de copyright
© 2022. The Author(s).
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