Passive sampling of environmental DNA in aquatic environments using 3D-printed hydroxyapatite samplers.
3D-printing
DNA adsorption
environmental DNA
hydroxyapatite
passive sampling
Journal
Molecular ecology resources
ISSN: 1755-0998
Titre abrégé: Mol Ecol Resour
Pays: England
ID NLM: 101465604
Informations de publication
Date de publication:
Aug 2022
Aug 2022
Historique:
revised:
16
02
2022
received:
24
05
2021
accepted:
17
02
2022
pubmed:
27
2
2022
medline:
7
7
2022
entrez:
26
2
2022
Statut:
ppublish
Résumé
The study of environmental DNA (eDNA) released by aquatic organisms in their habitat offers a fast, noninvasive and sensitive approach to monitor their presence. Common eDNA sampling methods such as water filtration and DNA precipitation are time-consuming, require difficult-to-handle equipment and partially integrate eDNA signals. To overcome these limitations, we created the first proof of concept of a passive, 3D-printed and easy-to-use eDNA sampler. We designed the samplers from hydroxyapatite (HAp samplers), a natural mineral with a high DNA adsorption capacity. The porous structure and shape of the samplers were designed to optimize DNA adsorption and facilitate their handling in the laboratory and in the field. Here we show that HAp samplers can efficiently collect genomic DNA in controlled set-ups, but can also collect animal eDNA under controlled and natural conditions with yields similar to conventional methods. However, we also observed large variations in the amount of DNA collected even under controlled conditions. A better understanding of the DNA-hydroxyapatite interactions on the surface of the samplers is now necessary to optimize eDNA adsorption and to allow the development of a reliable, easy-to-use and reusable eDNA sampling tool.
Identifiants
pubmed: 35218316
doi: 10.1111/1755-0998.13604
doi:
Substances chimiques
DNA, Environmental
0
DNA
9007-49-2
Durapatite
91D9GV0Z28
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2158-2170Subventions
Organisme : Office Française de la Biodiversité (OFB)
Organisme : Agence Française de la Recherche Technologique (ANRT)
Organisme : CNRS Mission pour les Initiatives Transverses et Interdisciplinaires (project XLIFE CAPTAS)
Organisme : Graduate School H2O'Lyon (ANR-17-EURE-0018)
Organisme : Eurofins Hydrobiologie France
Organisme : Université de Lyon (UdL)
Informations de copyright
© 2022 John Wiley & Sons Ltd.
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