An Engineered Cytidine Deaminase for Biocatalytic Production of a Key Intermediate of the Covid-19 Antiviral Molnupiravir.


Journal

Journal of the American Chemical Society
ISSN: 1520-5126
Titre abrégé: J Am Chem Soc
Pays: United States
ID NLM: 7503056

Informations de publication

Date de publication:
09 03 2022
Historique:
pubmed: 1 3 2022
medline: 18 3 2022
entrez: 28 2 2022
Statut: ppublish

Résumé

The Covid-19 pandemic highlights the urgent need for cost-effective processes to rapidly manufacture antiviral drugs at scale. Here we report a concise biocatalytic process for Molnupiravir, a nucleoside analogue recently approved as an orally available treatment for SARS-CoV-2. Key to the success of this process was the development of an efficient biocatalyst for the production of

Identifiants

pubmed: 35224970
doi: 10.1021/jacs.1c11048
pmc: PMC8915250
doi:

Substances chimiques

Antiviral Agents 0
Hydroxylamines 0
Cytidine 5CSZ8459RP
Cytidine Deaminase EC 3.5.4.5
Uridine WHI7HQ7H85
molnupiravir YA84KI1VEW

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

3761-3765

Subventions

Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M017702/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M027023/1
Pays : United Kingdom

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Auteurs

Ashleigh J Burke (AJ)

Department of Chemistry, Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, Manchester M1 7DN, U.K.

William R Birmingham (WR)

Department of Chemistry, Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, Manchester M1 7DN, U.K.

Ying Zhuo (Y)

Department of Chemistry, Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, Manchester M1 7DN, U.K.

Thomas W Thorpe (TW)

Department of Chemistry, Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, Manchester M1 7DN, U.K.

Bruna Zucoloto da Costa (B)

Department of Chemistry, Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, Manchester M1 7DN, U.K.

Rebecca Crawshaw (R)

Department of Chemistry, Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, Manchester M1 7DN, U.K.

Ian Rowles (I)

Department of Chemistry, Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, Manchester M1 7DN, U.K.

James D Finnigan (JD)

Prozomix Ltd, Building 4, West End Ind. Estate, Haltwhistle NE49 9HA, U.K.

Carl Young (C)

Prozomix Ltd, Building 4, West End Ind. Estate, Haltwhistle NE49 9HA, U.K.

Gregory M Holgate (GM)

Sterling Pharma Solutions, Sterling Place, Dudley, Northumberland NE23 7QG, U.K.

Mark P Muldowney (MP)

Sterling Pharma Solutions, Sterling Place, Dudley, Northumberland NE23 7QG, U.K.

Simon J Charnock (SJ)

Prozomix Ltd, Building 4, West End Ind. Estate, Haltwhistle NE49 9HA, U.K.

Sarah L Lovelock (SL)

Department of Chemistry, Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, Manchester M1 7DN, U.K.

Nicholas J Turner (NJ)

Department of Chemistry, Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, Manchester M1 7DN, U.K.

Anthony P Green (AP)

Department of Chemistry, Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, Manchester M1 7DN, U.K.

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Classifications MeSH