Two wrongs make a right: heat stress reversion of a male-sterile Brassica napus line.
Brassica napus
chloroplasts
fertility restorer
heat stress
male sterility
thermotolerance
Journal
Journal of experimental botany
ISSN: 1460-2431
Titre abrégé: J Exp Bot
Pays: England
ID NLM: 9882906
Informations de publication
Date de publication:
02 06 2022
02 06 2022
Historique:
received:
28
11
2021
accepted:
28
02
2022
pubmed:
1
3
2022
medline:
7
6
2022
entrez:
28
2
2022
Statut:
ppublish
Résumé
Male-sterile lines play important roles in plant breeding to obtain hybrid vigour. The male sterility Lembke (MSL) system is a thermosensitive genic male sterility system of Brassica napus and is one of the main systems used in European rapeseed breeding. Interestingly, the MSL system shows high similarity to the 9012AB breeding system from China, including the ability to revert to fertile in high temperature conditions. Here we demonstrate that the MSL system is regulated by the same restorer of fertility gene BnaC9-Tic40 as the 9012AB system, which is related to the translocon at the inner envelope membrane of chloroplasts 40 (TIC40) from Arabidopsis. The male sterility gene of the MSL system was also identified to encode a chloroplast-localized protein which we call BnChimera; this gene shows high sequence similarity to the sterility gene previously described for the 9012AB system. For the first time, a direct protein interaction between BnaC9-Tic40 and the BnChimera could be demonstrated. In addition, we identify the corresponding amino acids that mediate this interaction and suggest how BnaC9-Tic40 acts as the restorer of fertility. Using an RNA-seq approach, the effects of heat treatment on the male fertility restoration of the C545 MSL system line were investigated. These data demonstrate that many pollen developmental pathways are affected by higher temperatures. It is hypothesized that heat stress reverses the male sterility via a combination of slower production of cell wall precursors in plastids and a slower flower development, which ultimately results in fertile pollen. The potential breeding applications of these results are discussed regarding the use of the MSL system in producing thermotolerant fertile plants.
Identifiants
pubmed: 35226731
pii: 6539845
doi: 10.1093/jxb/erac082
pmc: PMC9162185
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3531-3551Subventions
Organisme : German Excellence Initiative and the European Union Seventh Framework Program
ID : 291763
Organisme : Center for Integrated Protein Science Munich
Organisme : Helmholtz Society
ID : VG-NG-1039
Organisme : Deutsche Forschungsgemeinschaft
Informations de copyright
© The Author(s) 2022. Published by Oxford University Press on behalf of the Society for Experimental Biology.
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