The relationship between the aggrecan VNTR polymorphism and its content in lumbar intervertebral discs.
aggrecan
intervertebral disc
morphology
risk factor
Journal
Folia morphologica
ISSN: 1644-3284
Titre abrégé: Folia Morphol (Warsz)
Pays: Poland
ID NLM: 0374620
Informations de publication
Date de publication:
2023
2023
Historique:
received:
07
02
2022
accepted:
09
02
2022
medline:
30
5
2023
pubmed:
4
3
2022
entrez:
3
3
2022
Statut:
ppublish
Résumé
There is a specific polymorphism of the ACAN gene called the variable number of tandem repeats (VNTR), which is particularly interesting in the light of the development of intervertebral disc pathology and associated low back pain. The nucleus pulposus specimens were harvested from the L5/S1 intervertebral discs. The aggrecan content was determined using enzyme- linked immunosorbent assay (ELISA). Moreover, the VNTR polymorphism in the ACAN gene was evaluated. The genotyping of VNTR polymorphism in ACAN gene was successful in 94 tissue samples (48 homozygotes and 46 heterozygotes). The alleles were divided into four groups, in accordance with the number of tandem repeats in the ACAN gene. No difference between groups in the mean aggrecan mass nor in the mean degree of tissue moisture was observed. No relationship between the ACAN gene VNTR polymorphism and the aggrecan content was observed in studied Caucasian cadavers. Such a relationship may be a more complex phenomenon and exists in other populations.
Sections du résumé
BACKGROUND
BACKGROUND
There is a specific polymorphism of the ACAN gene called the variable number of tandem repeats (VNTR), which is particularly interesting in the light of the development of intervertebral disc pathology and associated low back pain.
MATERIALS AND METHODS
METHODS
The nucleus pulposus specimens were harvested from the L5/S1 intervertebral discs. The aggrecan content was determined using enzyme- linked immunosorbent assay (ELISA). Moreover, the VNTR polymorphism in the ACAN gene was evaluated.
RESULTS
RESULTS
The genotyping of VNTR polymorphism in ACAN gene was successful in 94 tissue samples (48 homozygotes and 46 heterozygotes). The alleles were divided into four groups, in accordance with the number of tandem repeats in the ACAN gene. No difference between groups in the mean aggrecan mass nor in the mean degree of tissue moisture was observed.
CONCLUSIONS
CONCLUSIONS
No relationship between the ACAN gene VNTR polymorphism and the aggrecan content was observed in studied Caucasian cadavers. Such a relationship may be a more complex phenomenon and exists in other populations.
Identifiants
pubmed: 35239182
pii: VM/OJS/J/88305
doi: 10.5603/FM.a2022.0019
doi:
Substances chimiques
Aggrecans
0
ACAN protein, human
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM