Molecular identification and characterization of measles virus genotypes circulating in Afghanistan, 2008-2018.


Journal

Journal of medical virology
ISSN: 1096-9071
Titre abrégé: J Med Virol
Pays: United States
ID NLM: 7705876

Informations de publication

Date de publication:
07 2022
Historique:
revised: 17 02 2022
received: 07 01 2022
accepted: 04 03 2022
pubmed: 10 3 2022
medline: 21 5 2022
entrez: 9 3 2022
Statut: ppublish

Résumé

The measles virus (MV) remains a leading cause of morbidity and mortality in children under 5 years of age. Molecular identification of circulating wild-type MV) strains is a vital component of the measles elimination program. We received 159 oral swab samples from Afghanistan during 2008-2018. Viral RNA was extracted, followed by one-step RT-PCR and positive amplicons were subject to sequencing for genotype identification. Out of 159 total samples, 52% (83/159) were detected positive by RT-PCR. Genotype D4 was identified from 2.4% (2/83), genotype H1, 4.8% (4/83), and genotype B3, 92.7% (77/83) cases, respectively.

Identifiants

pubmed: 35261036
doi: 10.1002/jmv.27707
doi:

Substances chimiques

RNA, Viral 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

3448-3452

Subventions

Organisme : World Health Organization
ID : 001
Pays : International

Informations de copyright

© 2022 Wiley Periodicals LLC.

Références

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Auteurs

Muhammad S Rana (MS)

Department of Virology, National Institute of Health, Islamabad, Pakistan.

Muhammad Usman (M)

Department of Virology, National Institute of Health, Islamabad, Pakistan.

Aamer Ikram (A)

Department of Virology, National Institute of Health, Islamabad, Pakistan.

Muhammad M Alam (MM)

Department of Virology, National Institute of Health, Islamabad, Pakistan.

Muhammad Salman (M)

Department of Virology, National Institute of Health, Islamabad, Pakistan.

Syed S Z Zaidi (SSZ)

Department of Virology, National Institute of Health, Islamabad, Pakistan.

Massab Umair (M)

Department of Virology, National Institute of Health, Islamabad, Pakistan.

Nasir Stanikzai (N)

Department of Virology, Central Public Health Laboratory, Kabul, Afghanistan.

Abdul Shakoor (A)

Department of Surveillance, World Health Organization, Kabul, Afghanistan.

Fazel Afghan (F)

Department of Virology, Central Public Health Laboratory, Kabul, Afghanistan.

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