Polymer-Carrying Ability of Actin Filaments Interacting with Myosin Motors in a Biological Motility System In Vitro.
PEGylation
bioinspired devices
cytoskeletal filaments
in vitro motility assays
molecular motors
Journal
Macromolecular bioscience
ISSN: 1616-5195
Titre abrégé: Macromol Biosci
Pays: Germany
ID NLM: 101135941
Informations de publication
Date de publication:
05 2022
05 2022
Historique:
revised:
10
02
2022
received:
24
11
2021
pubmed:
10
3
2022
medline:
18
5
2022
entrez:
9
3
2022
Statut:
ppublish
Résumé
The reconstituted motility system of actin-myosin is expected to be used in bioinspired transport devices, in which carried materials are attached to either moving actin filaments or walking myosin molecules. However, the dependence of the ability to transport on the size of the attached materials is still inadequately understood. Here, as carried materials, polyethylene glycols (PEGs) of various sizes are covalently bound to actin filaments, and the motility of PEG-attached filaments on a heavy meromyosin (HMM) immobilized on a glass surface is observed via fluorescence microscopy. Full attachment of 2 kDa PEG, with an approximately 2 nm gyration radius, decreases the velocity and fraction of moving actin filaments by approximately 10% relative to unattached filaments. For the 5 kDa PEG, the fraction of moving filaments is decreased by approximately 70% even when the filaments contain only 20% PEG-attached actin. The attachment of both sizes of PEGs suppresses the actin-activated ATPase activity at the same level. These results suggest that actin filaments can carry PEGs up to 2 kDa having the same size as actin monomers, while the rate of ATP hydrolysis is limited. The size dependence may provide a criterion for material delivery via actin filaments in nanotransport applications.
Identifiants
pubmed: 35261163
doi: 10.1002/mabi.202100471
doi:
Substances chimiques
Actins
0
Myosin Subfragments
0
Polymers
0
Myosins
EC 3.6.4.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2100471Informations de copyright
© 2022 Wiley-VCH GmbH.
Références
M. Richard, C. Blanch-Mercader, H. Ennomani, W. Cao, E. M. De La Cruz, J.-F. Joanny, F. Jülicher, L. Blanchoin, P. Martin, Proc. Natl. Acad. Sci. U. S. A 2019, 116, 14835.
A. Rai, D. Vang, M. Ritt, S. Sivaramakrishnan, J. Biol. Chem. 2021, 296, 100232.
W. Jung, A. P. Tabatabai, J. J. Thomas, S. M. A. Tabei, M. P. Murrell, T. Kim, Cytoskeletion 2019, 76, 517.
T. E. Sladewski, E. B. Krementsova, K. M. Trybus, Curr. Biol. 2016, 26, 2202.
A. T. Lombardo, S. R. Nelson, G. G. Kennedy, K. M. Trybus, S. Walcott, D. M. Warshaw, Proc. Natl. Acad. Sci. U. S. A. 2019, 116, 8326.
E. B. Krementsova, K. '. Furuta, K. Oiwa, K. M. Trybus, M. Y. Ali, J. Biol. Chem. 2017, 292, 10998.
M. G. L. Van Den Heuvel, C. Dekker, Science 2007, 317, 333.
J. J. Keya, A. M.d. R. Kabir, A. Kakugo, Biophys. Rev. 2020, 12, 401.
M. Persson, M. Gullberg, C. Tolf, A. M. Lindberg, A. Månsson, A. Kocer, PLoS One 2013, 8, e55931.
D. V. Nicolau, M. Lard, T. Korten, F. C. M. J. M. Van Delft, M. Persson, E. Bengtsson, A. Månsson, S. Diez, H. Linke, D. V. Nicolau, Proc. Natl. Acad. Sci. U. S. A. 2016, 113, 2591.
T. Korten, E. Tavkin, L. Scharrel, V. S. Kushwaha, S. Diez, Lab Chip 2018, 18, 3196.
M. Li, Y. Li, L. Jia, S. Li, M. Li, G. Yang, N. Liu, H. Ren, M. Mou, A. Zheng, H. Sun, Eur. J. Med. Chem. Rep. 2021, 3, 100009.
B. Scott, C. Marang, M. Woodward, E. P. Debold, Cytoskeletion 2021, 78, 185.
T. J. Stewart, V. Murthy, S. P. Dugan, J. E. Baker, J. Biol. Chem. 2021, 297, 101178.
S. J. Kron, J. A. Spudich, Proc. Natl. Acad. Sci. U. S. A. 1986, 83, 6272.
S. Ramachandran, K.-H. Ernst, G. D. Bachand, V. Vogel, H. Hess, Small 2006, 2, 330.
K.-E. Byun, K. Heo, S. Shim, H.-J. Choi, S. Hong, Small 2009, 5, 2659.
M. Lard, L. T. Siethoff, A. Månsson, H. Linke, Sci. Rep. 2013, 3, 1092.
C. B. O'connell, M. J. Tyska, M. S. Mooseker, Biochim. Biophys. Acta, Mol. Cell Res. 2007, 1773, 615.
H. Takatsuki, K. M. Rice, S. Asano, B. S. Day, M. Hino, K. Oiwa, R. Ishikawa, Y. Hiratsuka, T. Q. P. Uyeda, K. Kohama, E. R. Blough, Small 2010, 6, 452.
S. Iwabuchi, T. Takahashi, K. Hatori, Biochem. Biophys. Res. Commun. 2012, 422, 164.
H. Kaur, T. Das, R. Kumar, R. Ajore, L. M. Bharadwaj, BioSystems 2008, 92, 69.
S. Kumar, A. Mansson, Biotechnol. Adv. 2017, 35, 867.
D. S. Kudryashov, M. Phillips, E. Reisler, Biophys. J. 2004, 87, 1136.
T. Fujii, K. Namba, Nat. Commun. 2017, 8, 13969.
S. Z. Chou, T. D. Pollard, Nat. Commun. 2020, 11, 5897.
M. J. Roberts, M. D. Bentley, J. M. Harris, Adv. Drug Delivery Rev. 2012, 64, 116.
F. Oesterhelt, M. Rief, H. E. Gaub, New J. Phys. 1999, 1, 6.
R. Bhat, S. N. Timasheff, Protein Sci. 1992, 1, 1133.
P. A. Gurnev, C. B. Stanley, M. A. Aksoyoglu, K. Hong, V. A. Parsegian, S. M. Bezrukov, Macromolecules 2017, 50, 2477.
P. Guhathakurta, E. Prochniewicz, D. Thomas, Int. J. Mol. Sci. 2018, 19, 2628.
T. Oda, M. Iwasa, T. Aihara, Y. Maéda, A. Narita, Nature 2009, 457, 441.
T. Q. P. Uyeda, S. J. Kron, J. A. Spudich, J. Mol. Biol. 1990, 214, 699.
T. Iwase, Y. Sasaki, K. Hatori, Biochim. Biophys. Acta, Gen. Subj. 2017, 1861, 2717.
H. Lee, R. M. Venable, A. D. Mackerell, R. W. Pastor, Biophys. J. 2008, 95, 1590.
F. Zosel, A. Soranno, K. J. Buholzer, D. Nettels, B. Schuler, Proc. Natl. Acad. Sci. U. S. A. 2020, 117, 13480.
S. Munakata, K. Hatori, FEBS J. 2013, 280, 5875.
H. Oshima, T. Hayashi, M. Kinoshita, Biophys. J. 2016, 110, 2496.
I. D. C. Fraser, S. B. Marston, J. Biol. Chem. 1995, 270, 7836.
H. Mizuno, H. Honda, J. Biochem. 2006, 139, 289.
K. Tokuraku, R. Kurogi, R. Toya, T. Q. P. Uyeda, J. Mol. Biol. 2009, 386, 149.
R. Hirakawa, Y. Nishikawa, T. Q. P. Uyeda, K. Tokuraku, Cytoskeletion 2017, 74, 482.
J. Sakamaki, H. Honda, E. Imai, K. Hatori, K. Shimada, K. Matsuno, Biophys. Chem. 2003, 105, 59.
Y.i Jia, W. Dong, X. Feng, J. Li, J. Li, Nanoscale 2015, 7, 82.
C. Reuther, R. Catalano, A. Salhotra, V. Vemula, T. Korten, S. Diez, A. Månsson, New J. Phys. 2021, 23, 075007.
P. B. Lawrence, J. L. Price, Curr. Opin. Chem. Biol. 2016, 34, 88.
C. Rosin, M. Erlkamp, J. V. D. Ecken, S. Raunser, R. Winter, Biophys. J. 2014, 107, 2982.
M. Okami, Y. Sunada, K. Hatori, Int. J. Biol. Macromol. 2020, 163, 1147.
S. J. J. Kron, Y. Y. Y. Toyoshima, T. Q. P. Uyeda, J. A. A. Spudich, Methods Enzymol. 1991, 196, 399.
K. Hatori, T. Iwase, R. Wada, Arch. Biochem. Biophys. 2016, 603, 64.
R. Martinez-Neira, C. G. Dos Remedios, I. R. Mackay, J. Immunol. Methods 2008, 338, 63.