Enzyme-triggered antigen release enhances cross-presentation by dendritic cells.
Cathepsin S
Cross-presentation
Enzymatic
Nanoparticles
Ovalbumin
Journal
Nanomedicine : nanotechnology, biology, and medicine
ISSN: 1549-9642
Titre abrégé: Nanomedicine
Pays: United States
ID NLM: 101233142
Informations de publication
Date de publication:
06 2022
06 2022
Historique:
received:
20
12
2021
revised:
11
02
2022
accepted:
01
03
2022
pubmed:
15
3
2022
medline:
18
5
2022
entrez:
14
3
2022
Statut:
ppublish
Résumé
Nanoparticles hold great potential as vaccine carriers due to their highly versatile structure and the possibility to influence intracellular trafficking and antigen presentation by their design. In this study, we developed a nanoparticulate system with a new enzyme-triggered antigen release mechanism. For this novel approach, nanoparticle and model antigen ovalbumin were linked with a substrate of the early endosomal protease cathepsin S. This construct enabled the transfer of antigens delivered to bone marrow-derived dendritic cells from the endo-lysosomal compartments in the cytosol. Consecutively, our particles enhanced cross-presentation on dendritic cells and subsequently promoted a stronger activation of CD8
Identifiants
pubmed: 35283290
pii: S1549-9634(22)00031-4
doi: 10.1016/j.nano.2022.102545
pii:
doi:
Substances chimiques
Antigens
0
Vaccines
0
Ovalbumin
9006-59-1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
102545Informations de copyright
Copyright © 2022. Published by Elsevier Inc.