PotN represents a novel energy-state sensing PII subfamily, occurring in firmicutes.
Lentilactobacillus hilgardii
PII-like protein PotN
pyruvate/2-oxoglutarate/acetoin dehydrogenase AcoB
transcription factor GlnR
transporter of polyamines PotABCD
Journal
The FEBS journal
ISSN: 1742-4658
Titre abrégé: FEBS J
Pays: England
ID NLM: 101229646
Informations de publication
Date de publication:
09 2022
09 2022
Historique:
revised:
19
02
2022
received:
09
04
2021
accepted:
10
03
2022
pubmed:
15
3
2022
medline:
9
9
2022
entrez:
14
3
2022
Statut:
ppublish
Résumé
PII proteins are signal processor proteins that regulate the cellular metabolism of Bacteria, Archea and plant chloroplasts typically in response to the cellular nitrogen status. Here, we report the first biochemical characterization of a novel PII-like protein PotN from Lentilactobacillus hilgardii. PotN is encoded in an operon together with the potABCD genes, encoding the ABC transporter for spermidine/putrescine. Like canonical PII proteins, the native PotN has a trimeric structure and competitively binds ATP and ADP, but it does not bind 2-oxoglutarate. Immunoprecipitation and pull-down experiments revealed that PotN is associated in vivo with the transcriptional regulator GlnR and the beta-subunit of pyruvate/2-oxoglutarate/acetoin dehydrogenase AcoB. Moreover, in vitro assays revealed that the ATPase domain of PotA also is able to interact with PotN. Interaction analyses demonstrated that PotN preferentially associates with PotA in the ADP state, whereas it binds to GlnR at elevated ATP levels. This suggests that PotN regulates the transport of polyamines and GlnR-dependent gene expression in response to the energy availability for the cell.
Substances chimiques
Bacterial Proteins
0
Ketoglutaric Acids
0
PII Nitrogen Regulatory Proteins
0
Adenosine Diphosphate
61D2G4IYVH
Adenosine Triphosphate
8L70Q75FXE
Nitrogen
N762921K75
Banques de données
RefSeq
['QIR10170', 'AIS03498', 'SBW30903', 'CAB15669', 'SQE56252', 'AAC73553', 'AAC75606', 'AAF42322', 'AFK17834', 'AFK17836', 'WP_042707534', 'AEA46717', 'BAD79382', 'AEE8209']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
5305-5321Informations de copyright
© 2022 Federation of European Biochemical Societies.
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