Inhibition of retinoid X receptor improved the morphology, localization of desmosomal proteins and paracellular permeability in three-dimensional cultures of mouse keratinocytes.
desmosome
keratinocyte
retinoic acid
retinoid X receptor
three-dimensional culture
tight junction
Journal
Microscopy (Oxford, England)
ISSN: 2050-5701
Titre abrégé: Microscopy (Oxf)
Pays: England
ID NLM: 101595834
Informations de publication
Date de publication:
06 Jun 2022
06 Jun 2022
Historique:
received:
02
11
2021
revised:
04
02
2022
accepted:
16
02
2022
pubmed:
16
3
2022
medline:
9
6
2022
entrez:
15
3
2022
Statut:
ppublish
Résumé
Retinoic acid (RA) plays an important role in epithelial homeostasis and influences the morphology, proliferation, differentiation and permeability of epithelial cells. Mouse keratinocytes, K38, reconstituted non-keratinized stratified epithelium in three-dimensional (3D) cultures with serum, which contains retinol (a source of RA), but the morphology was different from in vivo epithelium. The formed epithelium was thick, with loosened cell-cell contacts. Here, we investigated whether the inhibition of RA receptor (RAR)/retinoid X receptor (RXR)-mediated signaling by an RXR antagonist, HX 531, improved K38 3D cultures in terms of morphology and intercellular junctions. The epithelium formed by 0.5 μM HX531 was thin, and the intercellular space was narrowed because of the restoration of the layer-specific distribution of desmoglein (DSG)-1, DSG3 and plakoglobin (PG). Moreover, the levels of desmosomal proteins and tight junction proteins, including DSG1, DSG2, DSG3, PG, claudin (CLDN)-1 and CLDN4 increased, but the adherens junction protein, E-cadherin, did not show any change. Furthermore, CLDN1 was recruited to occludin-positive cell-cell contacts in the superficial cells and transepithelial electrical resistance was increased. Therefore, K38 3D cultures treated with 0.5 μM HX531 provides a useful in vitro model to study intercellular junctions in the non-keratinized epithelium.
Identifiants
pubmed: 35289919
pii: 6530229
doi: 10.1093/jmicro/dfac007
pmc: PMC9169536
doi:
Substances chimiques
Benzoates
0
Biphenyl Compounds
0
Desmosomal Cadherins
0
Retinoid X Receptors
0
diazepinylbenzoic acid
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
152-160Informations de copyright
© The Author(s) 2022. Published by Oxford University Press on behalf of The Japanese Society of Microscopy.
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