Role of glycodeoxycholic acid to induce acute pancreatitis in Macaca nemestrina.
Macaca nemestrina
acute pancreatitis
experimental
glycodeoxycholic acid
Journal
Journal of medical primatology
ISSN: 1600-0684
Titre abrégé: J Med Primatol
Pays: Denmark
ID NLM: 0320626
Informations de publication
Date de publication:
06 2022
06 2022
Historique:
revised:
04
02
2022
received:
19
11
2021
accepted:
26
02
2022
pubmed:
21
3
2022
medline:
28
4
2022
entrez:
20
3
2022
Statut:
ppublish
Résumé
Acute pancreatitis exhibits a rapid clinical progression which makes it difficult to observe in human; hence, an experimental animal model is needed. This preliminary study performed an induction of acute pancreatitis using glycodeoxycholic acid (GDOC) in an experimental macaque model. GDOC injections (initial dose of 11.20 mg/kg) were administered in an escalating manner at specific time points. The injection was given along the bilio-pancreatic duct, followed by measurement of vital signs, serum amylase-lipase, TNF-α, procalcitonin, oxidative stress parameters, and microscopic and macroscopic findings. The results indicated that acute pancreatitis occurred following induction with low-dose GDOC. Serum amylase and lipase levels increased with subsequent GDOC injections. Blood pressure and heart rate were elevated, indicating abdominal pain. Changes in TNF-α, procalcitonin, and oxidative stress values showed active inflammation. We observed histologic features of pancreatitis and as the dose increased, vasodilation of the splanchnic vasculatures was observed. Small dose GDOC injection in the bilio-pancreatic duct may have a role to induce acute pancreatitis in Macaca nemestrina.
Sections du résumé
BACKGROUND
Acute pancreatitis exhibits a rapid clinical progression which makes it difficult to observe in human; hence, an experimental animal model is needed. This preliminary study performed an induction of acute pancreatitis using glycodeoxycholic acid (GDOC) in an experimental macaque model.
METHODS
GDOC injections (initial dose of 11.20 mg/kg) were administered in an escalating manner at specific time points. The injection was given along the bilio-pancreatic duct, followed by measurement of vital signs, serum amylase-lipase, TNF-α, procalcitonin, oxidative stress parameters, and microscopic and macroscopic findings.
RESULTS
The results indicated that acute pancreatitis occurred following induction with low-dose GDOC. Serum amylase and lipase levels increased with subsequent GDOC injections. Blood pressure and heart rate were elevated, indicating abdominal pain. Changes in TNF-α, procalcitonin, and oxidative stress values showed active inflammation. We observed histologic features of pancreatitis and as the dose increased, vasodilation of the splanchnic vasculatures was observed.
CONCLUSIONS
Small dose GDOC injection in the bilio-pancreatic duct may have a role to induce acute pancreatitis in Macaca nemestrina.
Identifiants
pubmed: 35306662
doi: 10.1111/jmp.12577
pmc: PMC9310849
doi:
Substances chimiques
Procalcitonin
0
Tumor Necrosis Factor-alpha
0
Glycodeoxycholic Acid
360-65-6
Lipase
EC 3.1.1.3
Amylases
EC 3.2.1.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
134-142Informations de copyright
© 2022 The Authors. Journal of Medical Primatology published by John Wiley & Sons Ltd.
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