Development of two species of the Trypanosoma theileri complex in tabanids.
Deerflies
Horseflies
Life cycle
Trypanosomes
Vector
Journal
Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774
Informations de publication
Date de publication:
21 Mar 2022
21 Mar 2022
Historique:
received:
07
12
2021
accepted:
18
02
2022
entrez:
22
3
2022
pubmed:
23
3
2022
medline:
24
3
2022
Statut:
epublish
Résumé
Trypanosoma theileri species complex includes parasites of Bovidae (cattle, sheep, goat, etc.) and Cervidae (deer) transmitted mainly by Tabanidae (horse flies and deerflies) and keds (Hippoboscidae). While morphological discrimination of species is challenging, two big clades, TthI and TthII, each containing parasites isolated from bovids and cervids, have been identified phylogenetically. To date, the development in the vector has been studied in detail only for the ked-transmitted sheep parasite T. melophagium (TthII), while the fate of trypanosomes in tabanids was described only briefly by light microscopy. We collected infected tabanids of various species and identified trypanosomes by molecular phylogenetic analysis. The morphology and development of trypanosomes was studied using the combination of statistical analyses as well as light and electron microscopy. Two trypanosome species belonging to both TthI and TthII clades of the T. theileri complex were identified. The phylogenetic position of these two trypanosomes suggests that they parasitize deer. Both species were indiscernible by morphology in the vector and showed the same development in its intestine. In contrast to the previously described development of T. melophagium, both trypanosomes of tabanids only transiently infected midgut and settled mainly in the ileum, while pylorus and rectum were neglected. Meanwhile, the flagellates developing in the tabanid ileum (pyriform epimastigotes and metacyclic trypomastigotes) showed similarities to the corresponding stages in T. melophagium by morphology, mode of attachment to the host cuticle and formation of the fibrillar matrix surrounding the mass of developing parasites. In addition, for the first time to our knowledge we documented extraintestinal stages in these trypanosomes, located in the space between the epithelium and circular muscles. The development of different species of flagellates of the T. theileri complex in their insect vectors shows many similarities, which can be explained not only by their common origin, but also the same transmission mode, i.e. contamination of the oral mucosa with the gut content released after squashing the insect either by tongue or teeth. The observed differences (concerning primarily the distribution of developmental stages in the intestine) are associated rather with the identity of vectors than the phylogenetic position of parasites.
Sections du résumé
BACKGROUND
BACKGROUND
Trypanosoma theileri species complex includes parasites of Bovidae (cattle, sheep, goat, etc.) and Cervidae (deer) transmitted mainly by Tabanidae (horse flies and deerflies) and keds (Hippoboscidae). While morphological discrimination of species is challenging, two big clades, TthI and TthII, each containing parasites isolated from bovids and cervids, have been identified phylogenetically. To date, the development in the vector has been studied in detail only for the ked-transmitted sheep parasite T. melophagium (TthII), while the fate of trypanosomes in tabanids was described only briefly by light microscopy.
METHODS
METHODS
We collected infected tabanids of various species and identified trypanosomes by molecular phylogenetic analysis. The morphology and development of trypanosomes was studied using the combination of statistical analyses as well as light and electron microscopy.
RESULTS
RESULTS
Two trypanosome species belonging to both TthI and TthII clades of the T. theileri complex were identified. The phylogenetic position of these two trypanosomes suggests that they parasitize deer. Both species were indiscernible by morphology in the vector and showed the same development in its intestine. In contrast to the previously described development of T. melophagium, both trypanosomes of tabanids only transiently infected midgut and settled mainly in the ileum, while pylorus and rectum were neglected. Meanwhile, the flagellates developing in the tabanid ileum (pyriform epimastigotes and metacyclic trypomastigotes) showed similarities to the corresponding stages in T. melophagium by morphology, mode of attachment to the host cuticle and formation of the fibrillar matrix surrounding the mass of developing parasites. In addition, for the first time to our knowledge we documented extraintestinal stages in these trypanosomes, located in the space between the epithelium and circular muscles.
CONCLUSIONS
CONCLUSIONS
The development of different species of flagellates of the T. theileri complex in their insect vectors shows many similarities, which can be explained not only by their common origin, but also the same transmission mode, i.e. contamination of the oral mucosa with the gut content released after squashing the insect either by tongue or teeth. The observed differences (concerning primarily the distribution of developmental stages in the intestine) are associated rather with the identity of vectors than the phylogenetic position of parasites.
Identifiants
pubmed: 35313955
doi: 10.1186/s13071-022-05212-y
pii: 10.1186/s13071-022-05212-y
pmc: PMC8935851
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
95Subventions
Organisme : Russian Science Foundation
ID : 21-14-00191
Organisme : European Regional Development Fund
ID : CZ.02.1.01/16_019/0000759
Informations de copyright
© 2022. The Author(s).
Références
J Eukaryot Microbiol. 2012 Mar-Apr;59(2):134-44
pubmed: 22168919
J Parasitol. 1975 Feb;61(1):17-23
pubmed: 1117365
Parasitol Res. 2015 Mar;114(3):1071-8
pubmed: 25544706
Trop Anim Health Prod. 1995 May;27(2):93-4
pubmed: 7652945
Onderstepoort J Vet Res. 2004 Dec;71(4):251-6
pubmed: 15732451
Parasitology. 2021 Nov;148(13):1636-1647
pubmed: 34311794
PLoS One. 2018 Sep 27;13(9):e0204467
pubmed: 30261003
Nat Methods. 2017 Jun;14(6):587-589
pubmed: 28481363
Parasitol Res. 2019 Jun;118(6):1927-1935
pubmed: 31055671
Stain Technol. 1960 Nov;35:313-23
pubmed: 13741297
Parasitology. 2006 Feb;132(Pt 2):215-24
pubmed: 16197590
Mol Biol Evol. 2013 Apr;30(4):772-80
pubmed: 23329690
Ticks Tick Borne Dis. 2021 Jan;12(1):101573
pubmed: 33007666
Vet Rec. 1993 Jun 26;132(26):653-6
pubmed: 8362471
Parasit Vectors. 2020 Jun 12;13(1):308
pubmed: 32532317
PLoS One. 2018 Apr 19;13(4):e0196052
pubmed: 29672618
Parasit Vectors. 2013 Aug 03;6(1):221
pubmed: 23915781
Acta Trop. 1978 Dec;35(4):319-28
pubmed: 32751
Parasitol Res. 1987;73(5):421-4
pubmed: 3658973
Cytobios. 1985;44(177-178):183-8
pubmed: 3833459
Acta Trop. 1975;32(1):65-74
pubmed: 239553
Z Parasitenkd. 1980;62(1):63-74
pubmed: 6994382
PLoS One. 2019 Apr 3;14(4):e0214484
pubmed: 30943229
Vet Parasitol. 1986 Jan;19(1-2):13-21
pubmed: 3962154
Vet Res Commun. 2000 Nov;24(7):471-5
pubmed: 11085467
Acta Trop. 1973 Apr;30(4):340-6
pubmed: 4149680
Rev Bras Parasitol Vet. 2018 Oct-Dec;27(4):579-583
pubmed: 30133593
Parasitol Int. 2010 Sep;59(3):318-25
pubmed: 20230907
Vet Parasitol. 2013 Oct 18;197(1-2):29-42
pubmed: 23683651
Infect Genet Evol. 2009 Jan;9(1):81-6
pubmed: 19027884
J Protozool. 1987 Feb;34(1):110-3
pubmed: 3572837
Int J Parasitol. 2015 Oct;45(12):741-8
pubmed: 26219672
PLoS One. 2020 Jan 16;15(1):e0227832
pubmed: 31945116
Syst Biol. 2012 May;61(3):539-42
pubmed: 22357727
Vet Rec. 2002 Jan 5;150(1):18-9
pubmed: 11817859
Mol Biochem Parasitol. 1996 Jan;75(2):197-205
pubmed: 8992318
Vet Res Forum. 2020 Spring;11(2):191-193
pubmed: 32782750
Biomed Res Int. 2018 Jul 15;2018:2597074
pubmed: 30112369
Ann N Y Acad Sci. 2008 Dec;1149:352-4
pubmed: 19120247
Mol Biol Evol. 2020 May 1;37(5):1530-1534
pubmed: 32011700
J Protozool. 1964 May;11:200-7
pubmed: 14179743
Parasitol Int. 2022 Feb;86:102476
pubmed: 34610467
J Eukaryot Microbiol. 1993 Nov-Dec;40(6):788-92
pubmed: 8292996
Parazitologiia. 2007 Mar-Apr;41(2):126-36
pubmed: 17578245
J Morphol. 1968 Jan;124(1):37-78
pubmed: 4297319
Folia Parasitol (Praha). 2020 Mar 25;67:
pubmed: 32350156
Rev Bras Parasitol Vet. 2008 Apr-Jun;17(2):113-4
pubmed: 18823581
Trends Parasitol. 2021 Jun;37(6):538-551
pubmed: 33714646
Parasit Vectors. 2018 Aug 2;11(1):447
pubmed: 30071897
Int J Parasitol. 2004 Nov;34(12):1393-404
pubmed: 15542100