The profile of urinary lipid metabolites in healthy dogs.


Journal

The Journal of veterinary medical science
ISSN: 1347-7439
Titre abrégé: J Vet Med Sci
Pays: Japan
ID NLM: 9105360

Informations de publication

Date de publication:
01 May 2022
Historique:
pubmed: 25 3 2022
medline: 4 5 2022
entrez: 24 3 2022
Statut: ppublish

Résumé

Polyunsaturated fatty acids, including arachidonic acid (AA), docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA), are converted to hundreds of lipid mediators by cyclooxygenases (COX), lipoxygenases (LOX), and cytochrome P450 (CYP), or through non-enzymatic processes, and they reflect inflammatory states of the body. We comprehensively analyzed lipid metabolites in dog urine using a liquid chromatograph-mass spectrometry (LC-MS/MS) to describe their metabolic characteristics. We detected 31 AA-derived metabolites, four EPA-derived metabolites, and a DHA-derived metabolite in all urine samples. Among AA-derived metabolites, 15, 5, 3, and 8 were generated by COX, LOX, CYP, and non-enzymatic oxidation respectively. This study will be the first step to use profiles of urinary lipid metabolites for better understanding and diagnosis of canine diseases.

Identifiants

pubmed: 35321995
doi: 10.1292/jvms.22-0020
pmc: PMC9177396
doi:

Substances chimiques

Eicosanoids 0
Docosahexaenoic Acids 25167-62-8
Arachidonic Acid 27YG812J1I
Cytochrome P-450 Enzyme System 9035-51-2
Eicosapentaenoic Acid AAN7QOV9EA
Prostaglandin-Endoperoxide Synthases EC 1.14.99.1

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

644-647

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Auteurs

Taiki Kida (T)

Department of Animal Radiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.

Arisa Yamazaki (A)

Department of Animal Radiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.

Koji Kobayashi (K)

Department of Animal Radiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.

Tatsuro Nakamura (T)

Department of Animal Radiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.

Takayuki Nakagawa (T)

Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.

Ryohei Nishimura (R)

Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.

Takahisa Murata (T)

Department of Animal Radiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.

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Classifications MeSH