Microsphere Peptide-Based Immunoassay for the Detection of Recombinant Bovine Somatotropin in Injection Preparations.
biorecognition
epitope mapping
peptide-based immunoassay
recombinant bovine somatotropin
suspension array
Journal
Biosensors
ISSN: 2079-6374
Titre abrégé: Biosensors (Basel)
Pays: Switzerland
ID NLM: 101609191
Informations de publication
Date de publication:
22 Feb 2022
22 Feb 2022
Historique:
received:
22
12
2021
revised:
27
01
2022
accepted:
11
02
2022
entrez:
24
3
2022
pubmed:
25
3
2022
medline:
7
4
2022
Statut:
epublish
Résumé
The use of peptides in immunoassays can be favored over the use of the full protein when more cost effective or less toxic approaches are needed, or when access to the full protein is lacking. Due to restricted access to recombinant bovine somatotropin (rbST), a protein enhancing growth and lactating performances of livestock, which use has been banned in the EU, Canada and Australia (amongst others), we developed a peptide-based biorecognition assay on an imaging planar array analyzer. For this, we identified the rbST epitope that is responsible for binding to the rbST-targeting monoclonal antibody 4H12 (MAb 4H12) to be
Identifiants
pubmed: 35323408
pii: bios12030138
doi: 10.3390/bios12030138
pmc: PMC8946150
pii:
doi:
Substances chimiques
Recombinant Proteins
0
Growth Hormone
9002-72-6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Dutch ministry of economic affairs
ID : 1277353901
Organisme : European Commission DG Health and Food Safety (EURL; Brussels, Belgium)
Références
J Mol Recognit. 2007 Sep-Oct;20(5):283-99
pubmed: 18074397
Analyst. 2013 Jan 7;138(1):111-7
pubmed: 22805655
J Dairy Sci. 2021 Apr;104(4):5069-5078
pubmed: 33663857
Methods Mol Biol. 2018;1785:239-248
pubmed: 29714023
Analyst. 2010 May;135(5):1147-52
pubmed: 20419268
Drug Test Anal. 2017 Apr;9(4):646-656
pubmed: 27302098
J Agric Food Chem. 2008 Aug 27;56(16):7044-8
pubmed: 18671399
Cell Discov. 2020 Feb 4;6:5
pubmed: 32025335
J Virol Methods. 2017 Sep;247:114-118
pubmed: 28545817
Proc Natl Acad Sci U S A. 1984 Jul;81(13):3998-4002
pubmed: 6204335
Biosens Bioelectron. 2013 Jun 15;44:77-84
pubmed: 23395726
Biosens Bioelectron. 2015 Aug 15;70:48-53
pubmed: 25794957
Vaccines (Basel). 2021 Mar 26;9(4):
pubmed: 33810325
Anal Chim Acta. 2011 Aug 26;700(1-2):189-93
pubmed: 21742132
Int J Biol Macromol. 2020 Oct 15;161:299-307
pubmed: 32464201
Dev Comp Immunol. 2013 Sep;41(1):68-76
pubmed: 23583523
PLoS One. 2017 Jul 17;12(7):e0181150
pubmed: 28715465
Drug Test Anal. 2012 May;4(5):362-7
pubmed: 21964757
Anal Chim Acta. 2007 Mar 14;586(1-2):239-45
pubmed: 17386718
Pediatr Allergy Immunol. 2020 Feb;31(2):158-166
pubmed: 31598994
PLoS One. 2015 Aug 26;10(8):e0134360
pubmed: 26308444
Ann Allergy Asthma Immunol. 2018 Mar;120(3):245-253
pubmed: 29378246
Malar J. 2018 Sep 6;17(1):324
pubmed: 30189885
Cell Mol Immunol. 2021 Mar;18(3):621-631
pubmed: 33483707
Sci Rep. 2019 Dec 5;9(1):18425
pubmed: 31804555
J Virol Methods. 2016 Sep;235:15-20
pubmed: 27166561
Clin Vaccine Immunol. 2016 Apr 04;23(4):294-303
pubmed: 26843487
AIDS Res Hum Retroviruses. 2018 Dec;34(12):1017-1027
pubmed: 30056751
Protein Sci. 2006 Nov;15(11):2558-67
pubmed: 17001032
Toxins (Basel). 2017 Dec 25;10(1):
pubmed: 29295601
Methods Mol Biol. 2011;726:77-94
pubmed: 21424444