A HepG2 Cell-Based Biosensor That Uses Stainless Steel Electrodes for Hepatotoxin Detection.
HepG2 cell line
adhesion
hepatotoxins
impedance biosensor
stainless steel
Journal
Biosensors
ISSN: 2079-6374
Titre abrégé: Biosensors (Basel)
Pays: Switzerland
ID NLM: 101609191
Informations de publication
Date de publication:
04 Mar 2022
04 Mar 2022
Historique:
received:
11
02
2022
revised:
27
02
2022
accepted:
02
03
2022
entrez:
24
3
2022
pubmed:
25
3
2022
medline:
7
4
2022
Statut:
epublish
Résumé
Humans are frequently exposed to environmental hepatotoxins, which can lead to liver failure. Biosensors may be the best candidate for the detection of hepatotoxins because of their high sensitivity and specificity, convenience, time-saving, low cost, and extremely low detection limit. To investigate suitability of HepG2 cells for biosensor use, different methods of adhesion on stainless steel surfaces were investigated, with three groups of experiments performed in vitro. Cytotoxicity assays, which include the resazurin assay, the neutral red assay (NR), and the Coomassie Brilliant Blue (CBB) assay, were used to determine the viability of HepG2 cells exposed to various concentrations of aflatoxin B1 (AFB1) and isoniazid (INH) in parallel. The viability of the HepG2 cells on the stainless steel surface was quantitatively and qualitatively examined with different microscopy techniques. A simple cell-based electrochemical biosensor was developed by evaluating the viability of the HepG2 cells on the stainless steel surface when exposed to various concentrations of AFB1 and INH by using electrochemical impedance spectroscopy (EIS). The results showed that HepG2 cells can adhere to the metal surface and could be used as part of the biosensor to determine simple hepatotoxic samples.
Identifiants
pubmed: 35323430
pii: bios12030160
doi: 10.3390/bios12030160
pmc: PMC8946082
pii:
doi:
Substances chimiques
Stainless Steel
12597-68-1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Slovenian Research Agency
ID : J1-2471
Organisme : Slovenian Research Agency
ID : J1-9162
Organisme : Slovenian Research Agency
ID : P1-0207
Organisme : Slovenian Research Agency
ID : P2-0046
Organisme : European Union´s Horizon 2020 research and innovation programme
ID : 739566
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