Effect of dimethyl alpha-ketoglutarate supplementation on the in vitro developmental competences of ovine oocytes.
Actins
/ pharmacology
Adenosine Triphosphate
Animals
Blastocyst
Dietary Supplements
Embryonic Development
Fertilization in Vitro
/ veterinary
Glutathione
/ pharmacology
Hydrogen Peroxide
/ pharmacology
In Vitro Oocyte Maturation Techniques
/ veterinary
Ketoglutaric Acids
Oocytes
Reactive Oxygen Species
/ pharmacology
Sheep
Sheep, Domestic
Swine
DMKG
Mitochondrial activity
Ovine oocytes
Oxidative stress
Reactive oxygen species production
Journal
Theriogenology
ISSN: 1879-3231
Titre abrégé: Theriogenology
Pays: United States
ID NLM: 0421510
Informations de publication
Date de publication:
May 2022
May 2022
Historique:
received:
01
09
2021
revised:
19
02
2022
accepted:
14
03
2022
pubmed:
25
3
2022
medline:
15
4
2022
entrez:
24
3
2022
Statut:
ppublish
Résumé
The supplementation of dimethyl alpha-ketoglutarate (DMKG) during the in vitro maturation (IVM) process has been shown to improve the in vitro developmental competences of porcine oocytes. Here, the effects of DMKG supplementation in IVM medium on the development competencies of ovine oocytes were investigated by analyzing the nuclear maturation rate to metaphase II (MII) stage, ATP synthesis, cortical granules (CGs) dynamic, F-actin polymerization, mitochondrial activity, mitochondrial damage, reactive oxygen species (ROS) production, intracellular glutathione (GSH) production, DNA damage, cellular apoptosis, fertilization capacity and blastocyst development potential of ovine oocytes. In addition, the oxidative stress damage model induced by H
Identifiants
pubmed: 35325641
pii: S0093-691X(22)00102-9
doi: 10.1016/j.theriogenology.2022.03.013
pii:
doi:
Substances chimiques
Actins
0
Ketoglutaric Acids
0
Reactive Oxygen Species
0
dimethyl alpha-ketoglutarate
0
Adenosine Triphosphate
8L70Q75FXE
Hydrogen Peroxide
BBX060AN9V
Glutathione
GAN16C9B8O
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
171-184Informations de copyright
Copyright © 2022 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors report no conflicts of interest in this work.