Enteric Viruses Nucleic Acids Distribution along the Digestive Tract of Rhesus Macaques with Idiopathic Chronic Diarrhea.
biogeography
idiopathic chronic diarrhea
viral metagenomics
virome
Journal
Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722
Informations de publication
Date de publication:
19 03 2022
19 03 2022
Historique:
received:
04
02
2022
revised:
11
03
2022
accepted:
17
03
2022
entrez:
26
3
2022
pubmed:
27
3
2022
medline:
15
4
2022
Statut:
epublish
Résumé
Idiopathic chronic diarrhea (ICD) is a little understood common clinical problem in captive rhesus macaques claiming 33% of medical culls unrelated to research. The eukaryotic virome in digestive tract tissues collected at necropsy from nine animals with ICD was characterized using viral metagenomics. We compared the distribution of viral reads in tissues and mucosal scrapings from the stomach, duodenum, jejunum, ileum, and the proximal, transverse, and distal colons. In situ hybridization (ISH) using viral probes were performed on fixed tissues. Deep sequencing revealed multiple viruses in the
Identifiants
pubmed: 35337045
pii: v14030638
doi: 10.3390/v14030638
pmc: PMC8951234
pii:
doi:
Substances chimiques
Nucleic Acids
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIH HHS
ID : P51 OD011107
Pays : United States
Organisme : NIH HHS
ID : R01AI123376
Pays : United States
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