Tryptophan Supplementation Increases the Production of Microbial-Derived AhR Agonists in an
SHIME
aryl hydrocarbon receptor
microbiota
tryptophan
tryptophan metabolites
Journal
Journal of agricultural and food chemistry
ISSN: 1520-5118
Titre abrégé: J Agric Food Chem
Pays: United States
ID NLM: 0374755
Informations de publication
Date de publication:
06 Apr 2022
06 Apr 2022
Historique:
pubmed:
29
3
2022
medline:
8
4
2022
entrez:
28
3
2022
Statut:
ppublish
Résumé
The aryl hydrocarbon receptor (AhR) plays an important role in intestinal homeostasis, and some microbial metabolites of tryptophan are known AhR agonists. In this study, we assessed the impact of tryptophan supplementation on the formation of tryptophan metabolites, AhR activation, and microbiota composition in the simulator of the human intestinal microbial ecosystem (SHIME). AhR activation, microbial composition, and tryptophan metabolites were compared during high tryptophan supplementation (4 g/L tryptophan), control, and wash-out periods. During tryptophan supplementation, the concentration of several tryptophan metabolites was increased compared to the control and wash-out period, but AhR activation by fermenter supernatant was significantly decreased. This was due to the higher levels of tryptophan, which was found to be an antagonist of AhR signaling. Tryptophan supplementation induced most microbial changes in the transverse colon including increased relative abundance of lactobacillus. We conclude that tryptophan supplementation leads to increased formation of AhR agonists in the colon.
Identifiants
pubmed: 35344652
doi: 10.1021/acs.jafc.1c04145
pmc: PMC8991005
doi:
Substances chimiques
Receptors, Aryl Hydrocarbon
0
Tryptophan
8DUH1N11BX
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3958-3968Références
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