3D bioassembly of cell-instructive chondrogenic and osteogenic hydrogel microspheres containing allogeneic stem cells for hybrid biofabrication of osteochondral constructs.


Journal

Biofabrication
ISSN: 1758-5090
Titre abrégé: Biofabrication
Pays: England
ID NLM: 101521964

Informations de publication

Date de publication:
12 04 2022
Historique:
received: 16 12 2021
accepted: 28 03 2022
pubmed: 29 3 2022
medline: 15 4 2022
entrez: 28 3 2022
Statut: epublish

Résumé

Recently developed modular bioassembly techniques hold tremendous potential in tissue engineering and regenerative medicine, due to their ability to recreate the complex microarchitecture of native tissue. Here, we developed a novel approach to fabricate hybrid tissue-engineered constructs adopting high-throughput microfluidic and 3D bioassembly strategies. Osteochondral tissue fabrication was adopted as an example in this study, because of the challenges in fabricating load bearing osteochondral tissue constructs with phenotypically distinct zonal architecture. By developing cell-instructive chondrogenic and osteogenic bioink microsphere modules in high-throughput, together with precise manipulation of the 3D bioassembly process, we successfully fabricated hybrid engineered osteochondral tissue

Identifiants

pubmed: 35344942
doi: 10.1088/1758-5090/ac61a3
doi:

Substances chimiques

Hydrogels 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© 2022 IOP Publishing Ltd.

Auteurs

Xiaolin Cui (X)

Christchurch Regenerative Medicine & Tissue Engineering Group (CReaTE), Department of Orthopaedic Surgery and Musculoskeletal Medicine, University of Otago, Christchurch 8011, New Zealand.
Department of Bone and Joint, The First Affiliated Hospital of Dalian Medical University, Dalian 116000, Liaoning, People's Republic of China.

Cesar R Alcala-Orozco (CR)

Christchurch Regenerative Medicine & Tissue Engineering Group (CReaTE), Department of Orthopaedic Surgery and Musculoskeletal Medicine, University of Otago, Christchurch 8011, New Zealand.

Kenzie Baer (K)

Christchurch Regenerative Medicine & Tissue Engineering Group (CReaTE), Department of Orthopaedic Surgery and Musculoskeletal Medicine, University of Otago, Christchurch 8011, New Zealand.

Jun Li (J)

Christchurch Regenerative Medicine & Tissue Engineering Group (CReaTE), Department of Orthopaedic Surgery and Musculoskeletal Medicine, University of Otago, Christchurch 8011, New Zealand.

Caroline A Murphy (CA)

Christchurch Regenerative Medicine & Tissue Engineering Group (CReaTE), Department of Orthopaedic Surgery and Musculoskeletal Medicine, University of Otago, Christchurch 8011, New Zealand.

Mitch Durham (M)

Christchurch Regenerative Medicine & Tissue Engineering Group (CReaTE), Department of Orthopaedic Surgery and Musculoskeletal Medicine, University of Otago, Christchurch 8011, New Zealand.

Gabriella Lindberg (G)

Christchurch Regenerative Medicine & Tissue Engineering Group (CReaTE), Department of Orthopaedic Surgery and Musculoskeletal Medicine, University of Otago, Christchurch 8011, New Zealand.

Gary J Hooper (GJ)

Christchurch Regenerative Medicine & Tissue Engineering Group (CReaTE), Department of Orthopaedic Surgery and Musculoskeletal Medicine, University of Otago, Christchurch 8011, New Zealand.

Khoon S Lim (KS)

Christchurch Regenerative Medicine & Tissue Engineering Group (CReaTE), Department of Orthopaedic Surgery and Musculoskeletal Medicine, University of Otago, Christchurch 8011, New Zealand.

Tim B F Woodfield (TBF)

Christchurch Regenerative Medicine & Tissue Engineering Group (CReaTE), Department of Orthopaedic Surgery and Musculoskeletal Medicine, University of Otago, Christchurch 8011, New Zealand.

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