Immunofluorescence Detection of Callose in Plant Tissue Sections.
Callose
Immunofluorescence microscopy
Monoclonal antibody
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
29
3
2022
pubmed:
30
3
2022
medline:
1
4
2022
Statut:
ppublish
Résumé
The accumulation of the cell wall component callose at plasmodesmata (PD) is crucial for the regulation of symplastic intercellular transport in plants. Here we describe protocols to fluorescently image callose in sectioned plant tissue using monoclonal antibodies. This protocol achieves high-resolution images by the fixation, embedding, and sectioning of plant material to expose internal cell walls. By using this protocol in combination with high-resolution confocal microscopy, we can detect PD callose in a variety of plant tissues and species.
Identifiants
pubmed: 35349139
doi: 10.1007/978-1-0716-2132-5_10
doi:
Substances chimiques
Glucans
0
callose
9064-51-1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
167-176Informations de copyright
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
Amsbury S, Kirk P, Benitez-Alfonso Y (2018) Emerging models on the regulation of intercellular transport by plasmodesmata-associated callose. J Exp Bot 69:105–115. https://doi.org/10.1093/jxb/erx337
doi: 10.1093/jxb/erx337
Cui W, Lee J-Y (2016) Arabidopsis callose synthases CalS1/8 regulate plasmodesmal permeability during stress. Nat Plants 2:16034. https://doi.org/10.1038/nplants.2016.34
doi: 10.1038/nplants.2016.34
pubmed: 27243643
Radford JE, Vesk M, Overall RL (1998) Callose deposition at plasmodesmata. Protoplasma 201:30–37. https://doi.org/10.1007/BF01280708
doi: 10.1007/BF01280708
Li W, Zhao Y, Liu C, Yao G, Wu S, Hou C, Zhang M, Wang D (2012) Callose deposition at plasmodesmata is a critical factor in restricting the cell-to-cell movement of Soybean mosaic virus. Plant Cell Rep 31:905–916. https://doi.org/10.1007/s00299-011-1211-y
doi: 10.1007/s00299-011-1211-y
pubmed: 22200865
Maule AJ, Gaudioso-Pedraza R, Benitez-Alfonso Y (2013) Callose deposition and symplastic connectivity are regulated prior to lateral root emergence. Commun Integr Biol 6:e26531. https://doi.org/10.4161/cib.26531
doi: 10.4161/cib.26531
Benitez-Alfonso Y, Faulkner C, Pendle A, Miyashima S, Helariutta Y, Maule A (2013) Symplastic intercellular connectivity regulates lateral root patterning. Dev Cell 26:136–147. https://doi.org/10.1016/j.devcel.2013.06.010
doi: 10.1016/j.devcel.2013.06.010
pubmed: 23850190
Knox JP (2008) Revealing the structural and functional diversity of plant cell walls. Curr Opin Plant Biol 11:308–313. https://doi.org/10.1016/j.pbi.2008.03.001
doi: 10.1016/j.pbi.2008.03.001
pubmed: 18424171
Meikle PJ, Bonig I, Hoogenraad NJ, Clarke AE, Stone BA (1991) The location of (1→3)-β-glucans in the walls of pollen tubes of Nicotiana alata using a (1→3)-β-glucan-specific monoclonal antibody. Planta 185:1–8. https://doi.org/10.1007/BF00194507
doi: 10.1007/BF00194507
pubmed: 24186272
Thomas J, Ingerfeld M, Nair H, Chauhan SS, Collings DA (2013) Pontamine fast scarlet 4B: a new fluorescent dye for visualising cell wall organisation in radiata pine tracheids. Wood Sci Technol 47:59–75. https://doi.org/10.1007/s00226-012-0483-x
doi: 10.1007/s00226-012-0483-x
Anderson CT, Carroll A, Akhmetova L, Somerville C (2010) Real-time imaging of cellulose reorientation during cell wall expansion in Arabidopsis roots. Plant Physiol 152:787–796. https://doi.org/10.1104/pp.109.150128
doi: 10.1104/pp.109.150128
pubmed: 19965966
pmcid: 2815888