Spatiotemporal Specific Blocking of Plasmodesmata by Callose Induction.
Callose
Induction
Plasmodesmata
Tissue-specific promoter
cals3m
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
29
3
2022
pubmed:
30
3
2022
medline:
1
4
2022
Statut:
ppublish
Résumé
Plasmodesmata are nanoscale cell wall channels connecting neighboring cells in plants. Intercellular trafficking of molecules via plasmodesmata plays important roles in various developmental processes and stress responses. The turnover of callose, a β-1,3-glucan polysaccharide depositing in the cell wall around plasmodesmata, controls the plasmodesmal permeability and symplasmic transport. Here, we describe a protocol for the spatiotemporally controlled induction of callose synthesis and plasmodesmata closure using the cals3m system. In this system, cals3m, a mutant CALLOSE SYNTHASE 3 (CALS3) gene, is driven by inducible tissue-specific promoters of interest. After appropriate induction by 17-β-estradiol, callose is overproduced within the corresponding specific domains, resulting in temporal closure of plasmodesmata at the cell-cell interfaces. This approach can be used to validate and dissect the function of plasmodesmata-mediated symplasmic communications.
Identifiants
pubmed: 35349155
doi: 10.1007/978-1-0716-2132-5_26
doi:
Substances chimiques
Glucans
0
callose
9064-51-1
Glucosyltransferases
EC 2.4.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
383-391Informations de copyright
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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