Potential novel markers in IBD and CRC diagnostics. Are MMP-19 and RAGE promising candidates?


Journal

Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia
ISSN: 1804-7521
Titre abrégé: Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub
Pays: Czech Republic
ID NLM: 101140142

Informations de publication

Date de publication:
Dec 2022
Historique:
received: 08 07 2021
accepted: 14 03 2022
pubmed: 31 3 2022
medline: 16 12 2022
entrez: 30 3 2022
Statut: ppublish

Résumé

Inflammatory bowel diseases and colorectal cancer are serious intestinal disorders with continuously increasing incidence. Many aspects of etiopathogenesis still remain unclear. There is an urgent need to improve early diagnostics and markers indicating the progression of the disease. The aim of our study was to analyze the expression of matrix metalloproteinase-19 (MMP-19), and the receptor for advanced glycation end-products (RAGE) in different cell subpopulations in inflammatory bowel diseases (IBD) and colorectal cancer (CRC) compared to the tissue in the vicinity of pathological processes. Expression of both markers in epithelium, macrophages and vessels were evaluated in IBD and CRC groups. They were detected using immunohistochemistry in paraffin sections. There were significant differences between the expression of MMP-19 on macrophages and vessels among healthy and cancer tissues. In both, macrophages and vessels were significantly lower levels in cancer tissues. The expression of MMP-19 on vessels was also significantly different between peritumoral and cancer tissues (higher levels in peritumoral tissue). RAGE expression in macrophages was significantly different between healthy and cancer tissues and between peritumoral and cancer tissues. There was significantly lower expression in cancer tissues than in healthy and peritumoral tissues. Expression of RAGE in vessels was significantly different just in the comparison of healthy and peritumoral tissues (higher levels in healthy tissues). Both markers seem to be promising potential auxiliary markers in IBD and CRC diagnostics. They can also improve evaluation of disease progression.

Identifiants

pubmed: 35352707
doi: 10.5507/bp.2022.014
doi:

Substances chimiques

matrix metalloproteinase 19 EC 3.4.24.-
Matrix Metalloproteinases, Secreted EC 3.4.24.-
Receptor for Advanced Glycation End Products 0
Biomarkers 0
AGER protein, human 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

380-385

Déclaration de conflit d'intérêts

The authors report no conflicts of interest in this work.

Références

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Auteurs

Pavel Horak (P)

Department of Surgery, 1st Faculty of Medicine, Charles University in Prague and University Hospital Bulovka, Prague, Czech Republic.

Petr Suhaj (P)

Department of Pathology and Molecular Medicine, 3.

Radoslav Matej (R)

Department of Pathology and Molecular Medicine, 3.
Department of Pathology, 3.

Monika Cervinkova (M)

Department of Surgery, 1st Faculty of Medicine, Charles University in Prague and University Hospital Bulovka, Prague, Czech Republic.
Department of Psychology, Faculty of Arts, Charles University in Prague, Prague, Czech Republic.

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Classifications MeSH