Hand2 delineates mesothelium progenitors and is reactivated in mesothelioma.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
30 03 2022
30 03 2022
Historique:
received:
01
12
2020
accepted:
04
03
2022
entrez:
31
3
2022
pubmed:
1
4
2022
medline:
2
4
2022
Statut:
epublish
Résumé
The mesothelium lines body cavities and surrounds internal organs, widely contributing to homeostasis and regeneration. Mesothelium disruptions cause visceral anomalies and mesothelioma tumors. Nonetheless, the embryonic emergence of mesothelia remains incompletely understood. Here, we track mesothelial origins in the lateral plate mesoderm (LPM) using zebrafish. Single-cell transcriptomics uncovers a post-gastrulation gene expression signature centered on hand2 in distinct LPM progenitor cells. We map mesothelial progenitors to lateral-most, hand2-expressing LPM and confirm conservation in mouse. Time-lapse imaging of zebrafish hand2 reporter embryos captures mesothelium formation including pericardium, visceral, and parietal peritoneum. We find primordial germ cells migrate with the forming mesothelium as ventral migration boundary. Functionally, hand2 loss disrupts mesothelium formation with reduced progenitor cells and perturbed migration. In mouse and human mesothelioma, we document expression of LPM-associated transcription factors including Hand2, suggesting re-initiation of a developmental program. Our data connects mesothelium development to Hand2, expanding our understanding of mesothelial pathologies.
Identifiants
pubmed: 35354817
doi: 10.1038/s41467-022-29311-7
pii: 10.1038/s41467-022-29311-7
pmc: PMC8967825
doi:
Substances chimiques
Basic Helix-Loop-Helix Transcription Factors
0
HAND2 protein, human
0
Hand2 protein, mouse
0
Transcription Factors
0
Zebrafish Proteins
0
hand2 protein, zebrafish
0
Banques de données
figshare
['10.6084/m9.figshare.13221053.v1']
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1677Informations de copyright
© 2022. The Author(s).
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