Permeability of P. gingivalis or its metabolic products through collagen and dPTFE membranes and their effects on the viability of osteoblast-like cells: an in vitro study.

Bone Regeneration Collagen Membranes, Artificial Osteoblasts / metabolism Permeability Porphyromonas gingivalis
Bacteria Guided bone regeneration Membrane Permeability dPTFE

Journal

Odontology
ISSN: 1618-1255
Titre abrégé: Odontology
Pays: Japan
ID NLM: 101134822

Informations de publication

Date de publication:
Oct 2022
Historique:
received: 23 08 2021
accepted: 15 03 2022
pubmed: 1 4 2022
medline: 14 9 2022
entrez: 31 3 2022
Statut: ppublish

Résumé

Membrane exposure is a widely reported and relatively common complication in Guided Bone Regeneration (GBR) procedures. The introduction of micro-porous dPTFE barriers, which are impervious to bacterial cells, could reduce the technique sensitivity to membrane exposure, even if there are no studies investigating the potential passage of bacterial metabolites through the barrier. Aim of this study was the in vitro evaluation of the permeability of three different GBR membranes (dPTFE, native and cross-linked collagen membranes) to Porphyromonas gingivalis; in those cases, where bacterial penetration could not be observed, another purpose was the analysis of the viability and differentiation capability of an osteosarcoma (U2OS) cell line in presence of bacteria eluate obtained through membrane percolation. A system leading to the percolation of P. gingivalis broth culture through the experimental membranes was arranged to assess the permeability to bacteria after 24 and 72 h of incubation. The obtained solution was then added to U2OS cell cultures which underwent, after 10 days of incubation, MTT and red alizarin essays. The dPTFE membrane showed resistance to bacterial penetration, while both types of collagen membranes were crossed by P. gingivalis after 24 h. The bacteria eluate filtered through dPTFE membrane didn't show any toxicity on U2OS cells. Results of this study demonstrate that dPTFE membranes can contrast the penetration of both P. gingivalis and its metabolites toxic for osteoblast-like cells. The toxicity analysis was not possible for the collagen membranes, since permeability to bacterial cells was observed within the first period of incubation.

Identifiants

DOI: 10.1007/s10266-022-00705-9 PMID: 35355145
pubmed: 35355145
doi: 10.1007/s10266-022-00705-9
pii: 10.1007/s10266-022-00705-9
doi:

Substances chimiques

Membranes, Artificial 0
Collagen 9007-34-5

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

710-718

Informations de copyright

© 2022. The Author(s), under exclusive licence to The Society of The Nippon Dental University.

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Auteurs

Giuseppina Nocca (G)

Dipartimento di Scienze biotecnologiche di base, cliniche intensivologiche e perioperatorie, Sezione di Biochimica, Università Cattolica del Sacro Cuore, Largo F. Vito, 1, 00168, Rome, Italy. giuseppina.nocca@unicatt.it.
Fondazione Policlinico Universitario A. Gemelli, IRCCS, Largo A. Gemelli 1, 00168, Rome, Italy. giuseppina.nocca@unicatt.it.

Pierfrancesco Filetici (P)

Divisione di chirurgia orale e implantologia, Dipartimento Testa-collo e organi di senso, Università Cattolica del Sacro Cuore, Largo F. Vito, 1, 00168, Rome, Italy. pfiletici@gmail.com.
UOC Chirurgia Odontostomatologica e Implantologia, Fondazione Policlinico Universitario A. Gemelli, IRCCS, Largo A. Gemelli 1, 00168, Rome, Italy. pfiletici@gmail.com.
ORCID: 0000-0001-5684-5051

Francesca Bugli (F)

Fondazione Policlinico Universitario A. Gemelli, IRCCS, Largo A. Gemelli 1, 00168, Rome, Italy.
Dipartimento di scienze biotecnologiche di base, cliniche intensivologiche e perioperatorie, Sezione di Microbiologia, Largo F. Vito, 1, 00168, Rome, Italy.

Alvaro Mordente (A)

Dipartimento di Scienze biotecnologiche di base, cliniche intensivologiche e perioperatorie, Sezione di Biochimica, Università Cattolica del Sacro Cuore, Largo F. Vito, 1, 00168, Rome, Italy.
Fondazione Policlinico Universitario A. Gemelli, IRCCS, Largo A. Gemelli 1, 00168, Rome, Italy.

Antonio D'Addona (A)

Divisione di chirurgia orale e implantologia, Dipartimento Testa-collo e organi di senso, Università Cattolica del Sacro Cuore, Largo F. Vito, 1, 00168, Rome, Italy.
UOC Chirurgia Odontostomatologica e Implantologia, Fondazione Policlinico Universitario A. Gemelli, IRCCS, Largo A. Gemelli 1, 00168, Rome, Italy.

Leonardo Dassatti (L)

Divisione di chirurgia orale e implantologia, Dipartimento Testa-collo e organi di senso, Università Cattolica del Sacro Cuore, Largo F. Vito, 1, 00168, Rome, Italy.
UOC Chirurgia Odontostomatologica e Implantologia, Fondazione Policlinico Universitario A. Gemelli, IRCCS, Largo A. Gemelli 1, 00168, Rome, Italy.

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Classifications MeSH

questionsmedicales.fr › Phénomènes biologiques › Régénération › Remodelage osseux › Régénération osseuse › Permeability of P. gingivalis or its metabolic...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Scléroprotéines › Protéines de la matrice extracellulaire › Collagène › Permeability of P. gingivalis or its metabolic...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomimétiques › Membrane artificielle › Permeability of P. gingivalis or its metabolic...
questionsmedicales.fr › Cellules › Cellules du tissu conjonctif › Ostéoblastes › Permeability of P. gingivalis or its metabolic...
questionsmedicales.fr › Phénomènes chimiques › Perméabilité › Permeability of P. gingivalis or its metabolic...
questionsmedicales.fr › Bactéries › Bactéries à Gram négatif › Bactéries anaérobies à Gram négatif › Bâtonnets à Gram négatif anaérobies droits, incurvés et spiralés › Bacteroidaceae › Porphyromonas › Porphyromonas gingivalis › Permeability of P. gingivalis or its metabolic...