Pharmacological and molecular characterization of the A-type muscarinic acetylcholine receptor from Anopheles gambiae.
Anopheles gambiae
calcium signalling
heterologous system
insects
muscarinic acetylcholine receptor
Journal
Insect molecular biology
ISSN: 1365-2583
Titre abrégé: Insect Mol Biol
Pays: England
ID NLM: 9303579
Informations de publication
Date de publication:
08 2022
08 2022
Historique:
revised:
03
02
2022
received:
24
09
2021
accepted:
24
03
2022
pubmed:
1
4
2022
medline:
8
7
2022
entrez:
31
3
2022
Statut:
ppublish
Résumé
Muscarinic acetylcholine receptors (mAChRs) which are G protein-coupled receptors play key roles in insect physiology. Whereas vertebrate mAChRs are important targets for pharmaceutical drugs, insect mAChRs are under-exploited by the agro-chemical industry. Moreover, insect mAChRs have been less well studied than their vertebrate counterparts. Their critical functions mean that a better knowledge of the insect mAChRs is crucial for the effort to develop a new molecular-level strategy for insect pest management. Almost all insects possess three mAChRs named A, B and C which differ according to their coupling effector systems and their pharmacological profile. The aim of this study was to characterize the A-type mAChR (mAChR-A) from Anopheles gambiae which is the major vector of malaria in order to develop new strategies in pest management. In this paper, we reported that mAChR-A is more expressed in adult mosquitoes than in larvae. Furthermore, using calcium imaging recordings, we found that the An. gambiae mAChR-A expressed in Sf9 cells is activated by specific muscarinic agonists acetylcholine, muscarine and oxotremorine M and blocked by several mAChR antagonists. Moreover, using inhibitors of phosphoinositide pathway such as Gα
Substances chimiques
Insecticides
0
Receptors, Muscarinic
0
Acetylcholine
N9YNS0M02X
Calcium
SY7Q814VUP
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
497-507Informations de copyright
© 2022 Royal Entomological Society.
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