Studying Ctenophora WBR Using Mnemiopsis leidyi.

Ctenophore Husbandry Live imaging Mnemiopsis leidyi Surgeries Time-lapse Whole-body regeneration Wound healing

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2022
Historique:
entrez: 1 4 2022
pubmed: 2 4 2022
medline: 5 4 2022
Statut: ppublish

Résumé

Ctenophores, also known as comb jellies, are a clade of fragile holopelagic, carnivorous marine invertebrates, that represent one of the most ancient extant groups of multicellular animals. Ctenophores show a remarkable ability to regenerate in the adult form, being capable of replacing all body parts (i.e., whole-body regeneration) after loss/amputation. With many favorable experimental features (optical clarity, stereotyped cell lineage, multiple cell types), a full genome sequence available and their early branching phylogenetic position, ctenophores are well placed to provide information about the evolution of regenerative ability throughout the Metazoa. Here, we provide a collection of detailed protocols for use of the lobate ctenophore Mnemiopsis leidyi to study whole-body regeneration, including specimen collection, husbandry, surgical manipulation, and imaging techniques.

Identifiants

pubmed: 35359304
doi: 10.1007/978-1-0716-2172-1_5
pmc: PMC9761528
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

95-119

Informations de copyright

© 2022. The Author(s).

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Auteurs

Julia Ramon-Mateu (J)

The Whitney Laboratory for Marine Bioscience, St. Augustine, FL, USA. julia.ramon@imev-mer.fr.
Sorbonne Université, CNRS, Laboratoire de Biologie du Développement de Villefranche-sur-mer (LBDV), Villefranche-sur-mer, France. julia.ramon@imev-mer.fr.

Allison Edgar (A)

The Whitney Laboratory for Marine Bioscience, St. Augustine, FL, USA.

Dorothy Mitchell (D)

The Whitney Laboratory for Marine Bioscience, St. Augustine, FL, USA.

Mark Q Martindale (MQ)

The Whitney Laboratory for Marine Bioscience, St. Augustine, FL, USA. mqmartin@whitney.ufl.edu.

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Classifications MeSH