Automated Image Analyses of Glomerular Hypertrophy in a Mouse Model of Diabetic Nephropathy.
3D
CKD
LSFM
diabetes and the kidney
diabetes mellitus
diabetic nephropathies
glomeruli
hypertrophy
kidney glomerulus
lightsheet
microscopy
obesity
plant lectins
uninephrectomy
Journal
Kidney360
ISSN: 2641-7650
Titre abrégé: Kidney360
Pays: United States
ID NLM: 101766381
Informations de publication
Date de publication:
25 Jun 2020
25 Jun 2020
Historique:
received:
17
01
2020
accepted:
24
04
2020
entrez:
4
4
2022
pubmed:
1
5
2020
medline:
8
4
2022
Statut:
epublish
Résumé
Glomerular hypertrophy is a hallmark of kidney injury in metabolically induced renal diseases such as obesity-associated glomerulopathies and diabetic nephropathy (DN). Using light sheet fluorescent microscopy (LSFM) and 3D image analysis, we tested algorithms for automated and unbiased quantification of total glomerular numbers and individual glomerular volume in the uninephrectomized (UNx) db/db mouse model of DN. At 6 weeks after surgery, db/db and UNx db/db mice showed increased urine albumin-to-creatinine ratio (ACR) compared with db/+ control mice. Before euthanasia, glomeruli were labeled Overall, LSFM coupled with automated 3D histomorphometric analysis was demonstrated to be advantageous for unbiased assessment of glomerular volume and numbers in mouse whole-kidney samples. Furthermore, we showed that injection of fluorescently labeled lectin and albumin can be used as markers of nephron segments in the mouse kidneys, thus enabling functional assessment of kidney physiology, pathology, and pharmacology in preclinical rodent models of kidney disease.
Sections du résumé
Background
UNASSIGNED
Glomerular hypertrophy is a hallmark of kidney injury in metabolically induced renal diseases such as obesity-associated glomerulopathies and diabetic nephropathy (DN).
Methods
UNASSIGNED
Using light sheet fluorescent microscopy (LSFM) and 3D image analysis, we tested algorithms for automated and unbiased quantification of total glomerular numbers and individual glomerular volume in the uninephrectomized (UNx) db/db mouse model of DN.
Results
UNASSIGNED
At 6 weeks after surgery, db/db and UNx db/db mice showed increased urine albumin-to-creatinine ratio (ACR) compared with db/+ control mice. Before euthanasia, glomeruli were labeled
Conclusions
UNASSIGNED
Overall, LSFM coupled with automated 3D histomorphometric analysis was demonstrated to be advantageous for unbiased assessment of glomerular volume and numbers in mouse whole-kidney samples. Furthermore, we showed that injection of fluorescently labeled lectin and albumin can be used as markers of nephron segments in the mouse kidneys, thus enabling functional assessment of kidney physiology, pathology, and pharmacology in preclinical rodent models of kidney disease.
Identifiants
pubmed: 35368599
doi: 10.34067/KID.0001272019
pii: K3602019000127
pmc: PMC8809316
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
469-479Informations de copyright
Copyright © 2020 by the American Society of Nephrology.
Déclaration de conflit d'intérêts
All authors are current employees of Gubra ApS. L. Fink reports other from Novo Nordisk A/S outside the submitted work. J. Jelsing and N. Vrang are owners of Gubra. F. Sembach reports grants from Innovation Foundation outside the submitted work.
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