Lipid bilayer composition as a determinant of cancer cell sensitivity to tumoricidal protein-lipid complexes.
acyl chain unsaturation
lipidomics
plasma membrane
protein-lipid complexes
tumor specificity
Journal
BioFactors (Oxford, England)
ISSN: 1872-8081
Titre abrégé: Biofactors
Pays: Netherlands
ID NLM: 8807441
Informations de publication
Date de publication:
Sep 2022
Sep 2022
Historique:
received:
28
10
2021
accepted:
24
03
2022
pubmed:
8
4
2022
medline:
19
10
2022
entrez:
7
4
2022
Statut:
ppublish
Résumé
Complexes formed by the alpha1 N-terminal peptide of alpha-lactalbumin and oleic acid (alpha1-oleate) interact with lipid bilayers. Plasma membrane perturbations trigger tumor cell death but normal differentiated cells are more resistant, and their plasma membranes are less strongly affected. This study examined membrane lipid composition as a determinant of tumor cell reactivity. Bladder cancer tissue showed a higher abundance of unsaturated lipids enriched in phosphatidylcholine, PC (36:4) and PC (38:4), and sphingomyelin, SM (36:1) than healthy bladder tissue, where saturated lipids predominated and the lipid extracts from bladder cancer tissue inhibited the tumoricidal effect of the complex more effectively than healthy tissue extracts. Furthermore, unsaturated PC in solution inhibited tumor cell death, and the complex interacted with giant unilamellar vesicles formed by PC, confirming the affinity of alpha1-oleate for fluid membranes enriched in PC. Quartz Crystal Microbalance with dissipation monitoring (QCM-D) detected a preference of the complex for the liquid-disordered phase, suggesting that the insertion into PC-based membranes and the resulting membrane perturbations are influenced by membrane lipid saturation. The results suggest that the membrane lipid composition is functionally important and that specific unsaturated membrane lipids may serve as "recognition motifs" for broad-spectrum tumoricidal molecules such as alpha1-oleate.
Substances chimiques
Lipid Bilayers
0
Membrane Lipids
0
Phosphatidylcholines
0
Sphingomyelins
0
Tissue Extracts
0
Unilamellar Liposomes
0
Oleic Acid
2UMI9U37CP
Lactalbumin
9013-90-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1145-1159Subventions
Organisme : Agency for Science, Technology and Research
ID : IAF-ICP I1901E0040
Organisme : Cancerfonden
Organisme : Gunnar Nilssons Cancerstiftelse
Organisme : HJ Forssman Foundation for Medical Research
Organisme : Inga-Britt and Arne Lundberg Foundation
Organisme : Kungliga Fysiografiska Sällskapet i Lund
Organisme : Lunds Universitet
Organisme : Maggie Stephens Foundation
Organisme : Sharon D Lund foundation grant
Organisme : Soderberg Foundation
Organisme : Thorsten och Elsa Segerfalks Stiftelse
Organisme : National Research Foundation
ID : NRFSBP-P4
Organisme : Life Sciences Institute
Organisme : National University of Singapore
Organisme : Foundation for Medical Research
Organisme : Gunnar Nilsson Cancer Foundation
Organisme : Segerfalk Foundation
Organisme : Swedish Cancer Society
Organisme : Nanyang Technological University
Informations de copyright
© 2022 International Union of Biochemistry and Molecular Biology.
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