Effects of reduced gag cleavage efficiency on HIV-1 Gag-Pol package.

Fusion Proteins, gag-pol / genetics HIV-1 / genetics Leucine Zippers / genetics Virion Virus Assembly
Gag Gag cleavage Gag-Pol HIV-1 Protease Virus assembly

Journal

BMC microbiology
ISSN: 1471-2180
Titre abrégé: BMC Microbiol
Pays: England
ID NLM: 100966981

Informations de publication

Date de publication:
09 04 2022
Historique:
received: 11 10 2021
accepted: 23 03 2022
entrez: 9 4 2022
pubmed: 10 4 2022
medline: 13 4 2022
Statut: epublish

Résumé

HIV-1 pol, which encodes enzymes required for virus replication, is initially translated as a Gag-Pol fusion protein. Gag-Pol is incorporated into virions via interactions with Gag precursor Pr55 We engineered PR cleavage-blocking Gag mutations with the potential to significantly reduce Gag processing efficiency. Such mutations may mitigate the negative effects of enhanced PR activation on virus assembly and Gag-Pol packaging due to an RT dimerization enhancer or leucine zipper dimerization motif. When co-expressed with Pr55 Our data suggest that the amount of HIV-1 Gag-Pol or Pol viral incorporation is largely dependent on virus particle production, and that cleavage blocking in the Gag-Pol N-terminal Gag domain does not exert significant impacts on Pol packaging.

Sections du résumé

BACKGROUND
HIV-1 pol, which encodes enzymes required for virus replication, is initially translated as a Gag-Pol fusion protein. Gag-Pol is incorporated into virions via interactions with Gag precursor Pr55
RESULTS
We engineered PR cleavage-blocking Gag mutations with the potential to significantly reduce Gag processing efficiency. Such mutations may mitigate the negative effects of enhanced PR activation on virus assembly and Gag-Pol packaging due to an RT dimerization enhancer or leucine zipper dimerization motif. When co-expressed with Pr55
CONCLUSIONS
Our data suggest that the amount of HIV-1 Gag-Pol or Pol viral incorporation is largely dependent on virus particle production, and that cleavage blocking in the Gag-Pol N-terminal Gag domain does not exert significant impacts on Pol packaging.

Identifiants

DOI: 10.1186/s12866-022-02503-3 PMID: 35395730 PMC: PMC8994222
pubmed: 35395730
doi: 10.1186/s12866-022-02503-3
pii: 10.1186/s12866-022-02503-3
pmc: PMC8994222
doi:

Substances chimiques

Fusion Proteins, gag-pol 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

94

Informations de copyright

© 2022. The Author(s).

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Auteurs

Yi-Ru Lin (YR)

Institute of Clinical Medicine, National Yang Ming Chiao Tung University School of Medicine, 112, Taipei, Taiwan.

Shih-Ming Chu (SM)

Institute of Clinical Medicine, National Yang Ming Chiao Tung University School of Medicine, 112, Taipei, Taiwan.

Fu-Hsien Yu (FH)

Institute of Clinical Medicine, National Yang Ming Chiao Tung University School of Medicine, 112, Taipei, Taiwan.

Kuo-Jung Huang (KJ)

Division of Clinical Research, Department of Medical Research, Taipei Veterans General Hospital, Taipei, Taiwan.

Chin-Tien Wang (CT)

Institute of Clinical Medicine, National Yang Ming Chiao Tung University School of Medicine, 112, Taipei, Taiwan. chintien@nycu.edu.tw.
Division of Clinical Research, Department of Medical Research, Taipei Veterans General Hospital, Taipei, Taiwan. chintien@nycu.edu.tw.
ORCID: 0000-0003-2231-4492

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines virales › Protéines virales structurales › Protéines nucléocapside › Protéines du core viral › Produits du gène pol › Protéines de fusion gag-pol › Effects of reduced gag cleavage efficiency on...
questionsmedicales.fr › Virus › Virus à ARN › Retroviridae › Lentivirus › Lentivirus des primates › VIH (Virus de l'Immunodéficience Humaine) › VIH-1 (Virus de l'Immunodéficience Humaine de type 1) › Effects of reduced gag cleavage efficiency on...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Éléments structuraux des protéines › Glissières à leucine › Effects of reduced gag cleavage efficiency on...
questionsmedicales.fr › Structures virales › Virion › Effects of reduced gag cleavage efficiency on...
questionsmedicales.fr › Phénomènes microbiologiques › Phénomènes physiologiques viraux › Réplication virale › Assemblage viral › Effects of reduced gag cleavage efficiency on...