Characterization of an emerging porcine respirovirus 1 isolate in the US: A novel viral vector for expression of foreign antigens.
PRV1 infectious clone
PRV1 viral vector
Porcine parainfluenza virus 1
Porcine respirovirus 1
Journal
Virology
ISSN: 1096-0341
Titre abrégé: Virology
Pays: United States
ID NLM: 0110674
Informations de publication
Date de publication:
05 2022
05 2022
Historique:
received:
28
01
2022
revised:
29
03
2022
accepted:
29
03
2022
pubmed:
11
4
2022
medline:
29
4
2022
entrez:
10
4
2022
Statut:
ppublish
Résumé
Porcine respirovirus 1 (PRV1) is widely spread in many countries. In this study, we isolated an emgerging PRV1 strain (KS17-258) from a US swine farm. A full-length genome sequence of the virus was obtained, and the mRNA editing mechanism utilized for the expression of V/W proteins by P gene was confirmed. The virus shares 91.3-98% nucleotide sequence identity with the other PRV1 genomes reported previously. Phylogenetic analysis showed that KS17-258 forms a clade with the other US isolates. Infectious clone of the KS17-258 isolate was constructed, which was further explored as a viral vector to express enhanced green fluorescent protein (EGFP). The expression cassette of EGFP in the recombinant virus remained stable for 10 passages in cell culture. The availability of PRV1 infectious clone provides an important tool for study the basic PRV1 replication mechanisms. It also provides a novel platform for potential development of vectored vaccines against swine diseases.
Identifiants
pubmed: 35398774
pii: S0042-6822(22)00057-5
doi: 10.1016/j.virol.2022.03.013
pii:
doi:
Substances chimiques
DNA, Complementary
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
107-116Informations de copyright
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