Mitotic Maturation Compensates for Premature Centrosome Splitting and PCM Loss in Human
CEP135
SSX2IP
WDR8/WRAP73
centrosome
microtubule organization
mitosis
pericentriolar material
spindle
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
01 04 2022
01 04 2022
Historique:
received:
08
03
2022
revised:
29
03
2022
accepted:
30
03
2022
entrez:
12
4
2022
pubmed:
13
4
2022
medline:
14
4
2022
Statut:
epublish
Résumé
Centrosomes represent main microtubule organizing centers (MTOCs) in animal cells. Their duplication in S-phase enables the establishment of two MTOCs in M-phase that define the poles of the spindle and ensure equal distribution of chromosomes and centrosomes to the two daughter cells. While key functions of many centrosomal proteins have been addressed in RNAi experiments and chronic knockdown, knockout experiments with complete loss of function in all cells enable quantitative analysis of cellular phenotypes at all cell-cycle stages. Here, we show that the centriolar satellite proteins SSX2IP and WDR8 and the centriolar protein CEP135 form a complex before centrosome assembly in vertebrate oocytes and further functionally interact in somatic cells with established centrosomes. We present stable knockouts of
Identifiants
pubmed: 35406752
pii: cells11071189
doi: 10.3390/cells11071189
pmc: PMC8997944
pii:
doi:
Substances chimiques
CEP135 protein, human
0
Carrier Proteins
0
Cell Cycle Proteins
0
Microtubule-Associated Proteins
0
Proteins
0
SSX2IP protein, human
0
WRAP73 protein, human
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
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