Flow cytometry panels for immunophenotyping dairy cattle peripheral blood leukocytes.
Calves
Flow cytometry
Holstein cows
Peripartum
Peripheral blood
Journal
Veterinary immunology and immunopathology
ISSN: 1873-2534
Titre abrégé: Vet Immunol Immunopathol
Pays: Netherlands
ID NLM: 8002006
Informations de publication
Date de publication:
Jun 2022
Jun 2022
Historique:
received:
24
02
2022
revised:
23
03
2022
accepted:
31
03
2022
pubmed:
13
4
2022
medline:
14
5
2022
entrez:
12
4
2022
Statut:
ppublish
Résumé
Many aspects of the bovine immune system remain poorly characterized, which poses an obstacle to improving dairy cow health. Herein, we describe two flow cytometry panels that included antibodies against CD8α, CD4, TCR-δ, CD172α, CD14, MHCII, CD21, CD62L, and CD11b. These panels were used to characterize the phenotype of leukocyte subpopulations from the peripheral blood of 30-day old Holstein calves and Holstein cows at 260 d of gestation and calving. No leukocyte subset differences were found between the pre- and post-partum cows. However, calf leukocytes presented a higher proportion of CD3
Identifiants
pubmed: 35413495
pii: S0165-2427(22)00037-X
doi: 10.1016/j.vetimm.2022.110417
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
110417Informations de copyright
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