Long-range migration of centrioles to the apical surface of the olfactory epithelium.
cell biology
centriole
cilia
expansion microscopy
migration
mouse
neuroscience
olfaction
sensory neuron
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
14 04 2022
14 04 2022
Historique:
received:
02
10
2021
accepted:
13
04
2022
pubmed:
15
4
2022
medline:
6
5
2022
entrez:
14
4
2022
Statut:
epublish
Résumé
Olfactory sensory neurons (OSNs) in vertebrates detect odorants using multiple cilia, which protrude from the end of the dendrite and require centrioles for their formation. In mouse olfactory epithelium, the centrioles originate in progenitor cells near the basal lamina, often 50-100 μm from the apical surface. It is unknown how centrioles traverse this distance or mature to form cilia. Using high-resolution expansion microscopy, we found that centrioles migrate together, with multiple centrioles per group and multiple groups per OSN, during dendrite outgrowth. Centrioles were found by live imaging to migrate slowly, with a maximum rate of 0.18 µm/minute. Centrioles in migrating groups were associated with microtubule nucleation factors, but acquired rootletin and appendages only in mature OSNs. The parental centriole had preexisting appendages, formed a single cilium before other centrioles, and retained its unique appendage configuration in the mature OSN. We developed an air-liquid interface explant culture system for OSNs and used it to show that centriole migration can be perturbed
Identifiants
pubmed: 35420544
doi: 10.7554/eLife.74399
pii: 74399
pmc: PMC9064291
doi:
pii:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : K99 GM131024
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS082208
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM130286
Pays : United States
Informations de copyright
© 2022, Ching et al.
Déclaration de conflit d'intérêts
KC, JW, TS No competing interests declared
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