Highly efficient, processive and multifunctional recombinant endoglucanase RfGH5_4 from Ruminococcus flavefaciens FD-1 v3 for recycling lignocellulosic plant biomasses.
Endoglucanase
Multifunctional
Processive
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
01 Jun 2022
01 Jun 2022
Historique:
received:
08
02
2022
revised:
18
03
2022
accepted:
08
04
2022
pubmed:
15
4
2022
medline:
18
5
2022
entrez:
14
4
2022
Statut:
ppublish
Résumé
Gene encoding endoglucanase, RfGH5_4 from R. flavefaciens FD-1 v3 was cloned, expressed in Escherichia coli BL21(DE3) cells and purified. RfGH5_4 showed molecular size 41 kDa and maximum activity at pH 5.5 and 55 °C. It was stable between pH 5.0-8.0, retaining 85% activity and between 5 °C-45 °C, retaining 75% activity, after 60 min. RfGH5_4 displayed maximum activity (U/mg) against barley β-D-glucan (665) followed by carboxymethyl cellulose (450), xyloglucan (343), konjac glucomannan (285), phosphoric acid swollen cellulose (86), beechwood xylan (21.7) and carob galactomannan (16), thereby displaying the multi-functionality. Catalytic efficiency (mL.mg
Identifiants
pubmed: 35421411
pii: S0141-8130(22)00763-2
doi: 10.1016/j.ijbiomac.2022.04.059
pii:
doi:
Substances chimiques
lignocellulose
11132-73-3
Tegafur
1548R74NSZ
FD 1
62987-05-7
Cellulose
9004-34-6
Lignin
9005-53-2
Cellulase
EC 3.2.1.4
Carboxymethylcellulose Sodium
K679OBS311
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
801-813Informations de copyright
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