Measurement of procalcitonin in saliva of pigs: a pilot study.
Bacterial infections
Biomarkers
LPS
Meningitis
Porcine
Procalcitonin
Saliva
Sepsis
Turpentine oil
Journal
BMC veterinary research
ISSN: 1746-6148
Titre abrégé: BMC Vet Res
Pays: England
ID NLM: 101249759
Informations de publication
Date de publication:
15 Apr 2022
15 Apr 2022
Historique:
received:
19
01
2022
accepted:
08
04
2022
entrez:
16
4
2022
pubmed:
17
4
2022
medline:
20
4
2022
Statut:
epublish
Résumé
Procalcitonin (PCT) is a widely used biomarker of sepsis in human medicine and can have potential applications in the veterinary field. This study aimed to explore whether PCT could be measured in the saliva of pigs and whether its concentration changes in sepsis. Therefore, a specific assay was developed and analytically validated, and changes in PCT concentration were evaluated in two conditions: a) in an experimental model of sepsis produced by the administration of lipopolysaccharide (LPS) to pigs (n = 5), that was compared with a model of non-septic inflammation induced by turpentine oil (n = 4), and b) in healthy piglets (n = 11) compared to piglets with meningitis (n = 20), a disease that usually involves sepsis and whose treatment often requires large amounts of antibiotics in farms. The assay showed coefficients of variation within the recommended limits and adequate linearity after serial sample dilutions. The method's detection limit was set at 68 μg/L, and the lower limit of quantification was 414 μg/L. In the LPS experiment, higher concentrations of PCT were found after 24 h in the animals injected with LPS (mean = 5790 μg/L) compared to those treated with turpentine oil (mean = 2127 μg/L, P = 0.045). Also, animals with meningitis had higher concentrations of PCT (mean = 21515 μg/L) than healthy pigs (mean = 6096 μg/L, P value < 0.0001). According to these results, this assay could be potentially used as a tool for the non-invasive detection of sepsis in pigs, which is currently a topic of high importance due to antibiotic use restriction.
Sections du résumé
BACKGROUND
BACKGROUND
Procalcitonin (PCT) is a widely used biomarker of sepsis in human medicine and can have potential applications in the veterinary field. This study aimed to explore whether PCT could be measured in the saliva of pigs and whether its concentration changes in sepsis. Therefore, a specific assay was developed and analytically validated, and changes in PCT concentration were evaluated in two conditions: a) in an experimental model of sepsis produced by the administration of lipopolysaccharide (LPS) to pigs (n = 5), that was compared with a model of non-septic inflammation induced by turpentine oil (n = 4), and b) in healthy piglets (n = 11) compared to piglets with meningitis (n = 20), a disease that usually involves sepsis and whose treatment often requires large amounts of antibiotics in farms.
RESULTS
RESULTS
The assay showed coefficients of variation within the recommended limits and adequate linearity after serial sample dilutions. The method's detection limit was set at 68 μg/L, and the lower limit of quantification was 414 μg/L. In the LPS experiment, higher concentrations of PCT were found after 24 h in the animals injected with LPS (mean = 5790 μg/L) compared to those treated with turpentine oil (mean = 2127 μg/L, P = 0.045). Also, animals with meningitis had higher concentrations of PCT (mean = 21515 μg/L) than healthy pigs (mean = 6096 μg/L, P value < 0.0001).
CONCLUSIONS
CONCLUSIONS
According to these results, this assay could be potentially used as a tool for the non-invasive detection of sepsis in pigs, which is currently a topic of high importance due to antibiotic use restriction.
Identifiants
pubmed: 35428339
doi: 10.1186/s12917-022-03240-5
pii: 10.1186/s12917-022-03240-5
pmc: PMC9011990
doi:
Substances chimiques
Anti-Bacterial Agents
0
Biomarkers
0
Lipopolysaccharides
0
Procalcitonin
0
Turpentine
XJ6RUH0O4G
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
139Subventions
Organisme : Fundación Séneca
ID : 21293/FPI/19
Organisme : Universidad de Murcia
ID : Generational renewal to promote research
Organisme : Ministerio de Ciencia, Innovación y Universidades
ID : PID2019-105950RB-100
Informations de copyright
© 2022. The Author(s).
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