Methamphetamine can induce alteration of histopathology and sex determination gene expression through the oxidative stress pathway in the testes of human post-mortem.
Autopsy
Gene Expression
Humans
Interleukin-6
/ genetics
Male
Methamphetamine
/ toxicity
Oxidative Stress
Proliferating Cell Nuclear Antigen
/ metabolism
Reactive Oxygen Species
/ metabolism
SOXE Transcription Factors
/ genetics
Sex Determination Processes
Spermatogenesis
Testis
/ pathology
Tumor Necrosis Factor-alpha
/ genetics
human post-mortem
methamphetamine
sex determination genes
testis
Journal
Andrologia
ISSN: 1439-0272
Titre abrégé: Andrologia
Pays: Germany
ID NLM: 0423506
Informations de publication
Date de publication:
Aug 2022
Aug 2022
Historique:
revised:
13
03
2022
received:
19
11
2021
accepted:
04
04
2022
pubmed:
17
4
2022
medline:
15
7
2022
entrez:
16
4
2022
Statut:
ppublish
Résumé
Methamphetamine is a recreational drug that can be taken ingestion orally, injected, smoked or snorted. Methamphetamine abuse may lead to male infertility. The purpose of this study was to evaluate the long-term effects of methamphetamine abuse on the sex reprogramming of human post-mortem testis. Testes were collected from the autopsies of methamphetamine users (n = 10) and healthy males (reference group) (n = 10). They were then taken for stereological studies and RNA extraction to evaluate the expressions of PCNA, DMRT1, SOX8, c-Kit, TNF-α, IL6 and FOXL2 genes. In addition, Reactive Oxygen Species (ROS) level and Glutathione Disulfide (GSH) were assessed. Autopsied testicular samples of methamphetamine revealed a significant reduction in stereological parameters and histopathological findings, suggesting methamphetamine as a practical approach to prevention strategies in reproductive medicine that can disrupt spermatogenesis. Moreover, the results indicated the expressions of the genes involved in testis function and male-to-female genetic reprogramming (PCNA, DMRT1, SOX8, c-Kit, TNF-α, IL6 and FOXL2) (16) as well as in increasing inflammation (TNF-α and IL-6). The results also showed a high level of ROS and a decrease in GSH activity. The results of SOX9 immunohistochemistry indicated a significant decrease in the expression of SOX9 as well as in the number of Sertoli cells in the methamphetamine group. Overall, the results suggested that methamphetamine abuse caused spermatogenesis disruption and genetic reprogramming, probably through oxidative stress and changes in the expression of sex-determining genes.
Substances chimiques
Interleukin-6
0
Proliferating Cell Nuclear Antigen
0
Reactive Oxygen Species
0
SOX8 protein, human
0
SOXE Transcription Factors
0
Tumor Necrosis Factor-alpha
0
Methamphetamine
44RAL3456C
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e14441Subventions
Organisme : Shiraz University of Medical Sciences
Organisme : Shiraz University
Organisme : School of Medicine
Informations de copyright
© 2022 Wiley-VCH GmbH.
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