Rapalog-induced cell adhesion molecule inhibits mesoderm migration in Xenopus embryos by increasing frequency of adhesion to the ectoderm.
FKBP
FRB
Xenopus laevis
adhesion
boundary
mesoderm
migration
rapamycin
repulsion
Journal
Genes to cells : devoted to molecular & cellular mechanisms
ISSN: 1365-2443
Titre abrégé: Genes Cells
Pays: England
ID NLM: 9607379
Informations de publication
Date de publication:
Jun 2022
Jun 2022
Historique:
revised:
10
04
2022
received:
28
02
2022
accepted:
11
04
2022
pubmed:
20
4
2022
medline:
11
6
2022
entrez:
19
4
2022
Statut:
ppublish
Résumé
During the gastrula stage of Xenopus laevis, mesodermal cells migrate on the blastocoel roof (BCR) toward the animal pole. In this process, mesodermal cells directly adhere to the BCR via adhesion molecules, such as cadherins, which in turn trigger a repulsive reaction through factors such as Eph/ephrin. Therefore, the mesoderm and BCR repeatedly adhere to and detach from each other, and the frequency of this adhesion is thought to control mesoderm migration. Although knockdown of cadherin or Eph/ephrin causes severe gastrulation defects, these molecules have been reported to contribute not only to boundary formation but also to the internal function of each tissue. Therefore, it is possible that the defect caused by knockdown occurs due to tissue function abnormalities. To address this problem, we developed a method to specifically induce adhesion between different tissues using rapalog (an analog of rapamycin). When adhesion between the BCR and mesoderm was specifically enhanced by rapalog, mesoderm migration was strongly suppressed. Furthermore, we confirmed that rapalog significantly increased the frequency of adhesion between the two tissues. These results support the idea that the adhesion frequency controls mesoderm migration, and demonstrate that our method effectively enhances adhesion between specific tissues in vivo.
Substances chimiques
Cadherins
0
Cell Adhesion Molecules
0
Ephrins
0
MTOR Inhibitors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
436-450Subventions
Organisme : Japan Society for the Promotion of Science
ID : JP17H06259
Organisme : Japan Society for the Promotion of Science
ID : JP19H05801
Organisme : Precursory Research for Embryonic Science and Technology
ID : JPMJPR15F1
Informations de copyright
© 2022 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd.
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