Mechanosensation by endothelial PIEZO1 is required for leukocyte diapedesis.
Journal
Blood
ISSN: 1528-0020
Titre abrégé: Blood
Pays: United States
ID NLM: 7603509
Informations de publication
Date de publication:
21 07 2022
21 07 2022
Historique:
received:
29
10
2021
accepted:
27
03
2022
pubmed:
21
4
2022
medline:
26
7
2022
entrez:
20
4
2022
Statut:
ppublish
Résumé
The extravasation of leukocytes is a critical step during inflammation that requires the localized opening of the endothelial barrier. This process is initiated by the close interaction of leukocytes with various adhesion molecules such as ICAM-1 on the surface of endothelial cells. Here we reveal that mechanical forces generated by leukocyte-induced clustering of ICAM-1 synergize with fluid shear stress exerted by the flowing blood to increase endothelial plasma membrane tension and to activate the mechanosensitive cation channel PIEZO1. This leads to increases in [Ca2+]i and activation of downstream signaling events including phosphorylation of tyrosine kinases sarcoma (SRC) and protein tyrosine kinase 2 (PYK2), as well as of myosin light chain, resulting in opening of the endothelial barrier. Mice with endothelium-specific Piezo1 deficiency show decreased leukocyte extravasation in different inflammation models. Thus, leukocytes and the hemodynamic microenvironment synergize to mechanically activate endothelial PIEZO1 and subsequent downstream signaling to initiate leukocyte diapedesis.
Identifiants
pubmed: 35443048
pii: S0006-4971(22)00550-X
doi: 10.1182/blood.2021014614
pmc: PMC9305087
doi:
Substances chimiques
Ion Channels
0
Piezo1 protein, mouse
0
Intercellular Adhesion Molecule-1
126547-89-5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
171-183Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2022 by The American Society of Hematology.
Références
Curr Opin Cell Biol. 2020 Oct;66:69-78
pubmed: 32580115
Vet Pathol. 2013 Jan;50(1):7-22
pubmed: 23345459
Trends Immunol. 2017 Aug;38(8):606-615
pubmed: 28559148
iScience. 2018 Sep 28;7:180-190
pubmed: 30267679
Exp Mol Med. 2009 May 31;41(5):341-8
pubmed: 19307754
Cell. 2018 Dec 13;175(7):1769-1779.e13
pubmed: 30392960
Cell Rep. 2016 Nov 8;17(7):1739-1746
pubmed: 27829145
J Cell Biol. 1995 Mar;128(5):969-78
pubmed: 7533170
Cell Calcium. 2001 Jul;30(1):29-40
pubmed: 11396985
J Immunol. 2000 Sep 15;165(6):3375-83
pubmed: 10975856
Nat Immunol. 2004 Jul;5(7):713-20
pubmed: 15184895
Small GTPases. 2017 Jan 2;8(1):1-15
pubmed: 27715453
Am J Physiol Cell Physiol. 2012 Aug 15;303(4):C385-95
pubmed: 22648953
Atherosclerosis. 2020 Dec;315:102-110
pubmed: 32800322
J Cell Sci. 1998 Feb;111 ( Pt 4):443-53
pubmed: 9443894
Trends Biochem Sci. 2017 Jan;42(1):57-71
pubmed: 27743844
J Exp Med. 2011 Aug 1;208(8):1721-35
pubmed: 21788407
Cell Immunol. 2000 Jul 10;203(1):39-46
pubmed: 10915560
Proc Natl Acad Sci U S A. 2014 Jul 15;111(28):10347-52
pubmed: 24958852
J Cell Sci. 2014 Oct 15;127(Pt 20):4470-82
pubmed: 25107367
Elife. 2015 Dec 08;4:
pubmed: 26646186
Nat Rev Immunol. 2015 Nov;15(11):692-704
pubmed: 26471775
J Immunol. 2003 Dec 1;171(11):6135-44
pubmed: 14634129
J Biol Chem. 2005 Jun 3;280(22):21129-36
pubmed: 15778498
Nat Commun. 2016 Jan 20;7:10366
pubmed: 26785635
J Exp Med. 2015 Oct 19;212(11):1883-99
pubmed: 26392222
Cardiovasc Res. 2015 Aug 1;107(3):321-30
pubmed: 25990461
Immunol Rev. 2016 Sep;273(1):61-75
pubmed: 27558328
J Immunol. 2000 Jun 15;164(12):6487-94
pubmed: 10843706
Nature. 2014 Nov 13;515(7526):279-282
pubmed: 25119035
J Exp Med. 2021 Jan 4;218(1):
pubmed: 32970800
Cell Adh Migr. 2014;8(2):67-75
pubmed: 24621576
Am J Physiol Lung Cell Mol Physiol. 2017 Jun 1;312(6):L1003-L1017
pubmed: 28385807
Blood. 2001 Feb 1;97(3):660-8
pubmed: 11157482
F1000Res. 2016 Sep 14;5:
pubmed: 27703663
Front Immunol. 2018 Nov 28;9:2774
pubmed: 30546362
Nature. 2012 Feb 19;483(7388):176-81
pubmed: 22343900
Nat Chem. 2018 Nov;10(11):1118-1125
pubmed: 30150727
Cell Mol Bioeng. 2010 Mar 1;3(1):50-59
pubmed: 20862208
J Biol Chem. 2001 Mar 30;276(13):10224-8
pubmed: 11266438
Blood. 2000 Dec 1;96(12):3816-22
pubmed: 11090065
J Immunol. 2014 Apr 1;192(7):3390-8
pubmed: 24585879
Cell Rep. 2022 Feb 8;38(6):110342
pubmed: 35139384
Physiol Rev. 2019 Jul 1;99(3):1467-1525
pubmed: 31140373
J Exp Med. 2001 Dec 17;194(12):1699-709
pubmed: 11748272
Arterioscler Thromb Vasc Biol. 2007 Sep;27(9):1870-6
pubmed: 17585068
Oncogene. 2007 Feb 15;26(7):1067-77
pubmed: 16909109
Cell Mol Life Sci. 2016 Apr;73(8):1591-608
pubmed: 26794844
Nature. 2018 Feb 22;554(7693):487-492
pubmed: 29469092
Proc Natl Acad Sci U S A. 2018 Jan 2;115(1):133-138
pubmed: 29255056
J Immunol. 2006 Sep 15;177(6):4113-21
pubmed: 16951376
Nat Immunol. 2001 Jun;2(6):515-22
pubmed: 11376338
J Cell Biol. 1993 Mar;120(6):1371-80
pubmed: 8449983
Nat Rev Immunol. 2007 Sep;7(9):678-89
pubmed: 17717539
Nat Rev Mol Cell Biol. 2017 Dec;18(12):771-783
pubmed: 28974772
Immunity. 2014 Nov 20;41(5):694-707
pubmed: 25517612
J Immunol. 2007 Sep 15;179(6):4053-64
pubmed: 17785844
Pharmacol Rep. 2009 Jan-Feb;61(1):22-32
pubmed: 19307690
J Clin Invest. 2016 Dec 1;126(12):4527-4536
pubmed: 27797339
Curr Opin Cell Biol. 2020 Oct;66:11-18
pubmed: 32416466