Functional exploration of the glycoside hydrolase family GH113.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2022
2022
Historique:
received:
21
01
2022
accepted:
08
04
2022
entrez:
22
4
2022
pubmed:
23
4
2022
medline:
27
4
2022
Statut:
epublish
Résumé
β-Mannans are a heterogeneous group of polysaccharides with a common main chain of β-1,4-linked mannopyranoside residues. The cleavage of β-mannan chains is catalyzed by glycoside hydrolases called β-mannanases. In the CAZy database, β-mannanases are grouped by sequence similarity in families GH5, GH26, GH113 and GH134. Family GH113 has been under-explored so far with six enzymes characterized, all from the Firmicutes phylum. We undertook the functional characterization of 14 enzymes from a selection of 31 covering the diversity of the family GH113. Our observations suggest that GH113 is a family with specificity towards mannans, with variations in the product profiles and modes of action. We were able to assign mannanase and mannosidase activities to four out of the five clades of the family, increasing by 200% the number of characterized GH113 members, and expanding the toolbox for fine-tuning of mannooligosaccharides.
Identifiants
pubmed: 35452491
doi: 10.1371/journal.pone.0267509
pii: PONE-D-22-02049
pmc: PMC9032380
doi:
Substances chimiques
Mannans
0
Glycoside Hydrolases
EC 3.2.1.-
beta-Mannosidase
EC 3.2.1.25
Mannose
PHA4727WTP
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0267509Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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