Atlantic Salmon Mucins Inhibit
bacterial growth
biofilm
mucus
sialic acid
signaling
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
13 Apr 2022
13 Apr 2022
Historique:
received:
22
03
2022
revised:
11
04
2022
accepted:
12
04
2022
entrez:
23
4
2022
pubmed:
24
4
2022
medline:
27
4
2022
Statut:
epublish
Résumé
One of the most important bacterial diseases in salmonid aquaculture is furunculosis, caused by Aeromonas salmonicida. Bacterial communication through secreted autoinducer signals, quorum sensing, takes part in the regulation of gene expression in bacteria, influencing growth and virulence. The skin and mucosal surfaces, covered by a mucus layer, are the first point of contact between fish and bacteria. Mucins are highly glycosylated and are the main components of mucus. Here, we validate the Vibrio harveyi BB170 bioreporter assay for quantifying A. salmonicida quorum sensing and study the effects of Atlantic salmon mucins as well as mono- and disaccharides on the AI-2 levels of A. salmonicida. Atlantic salmon mucins from skin, pyloric ceca, proximal and distal intestine reduced A. salmonicida AI-2 levels. Among the saccharides abundant on mucins, fucose, N-acetylneuraminic acid and GlcNAcβ1-3Gal inhibited AI-2 A. salmonicida secretion. Removal of N-acetylneuraminic acid, which is the most abundant terminal residue on mucin glycans on Atlantic salmon mucins, attenuated the inhibitory effects on AI-2 levels of A. salmonicida. Deletion of A. salmonicida luxS abolished AI-2 production. In conclusion, Atlantic salmon mucins regulate A. salmonicida quorum sensing in a luxS and N-acetylneuraminic acid-dependent manner.
Identifiants
pubmed: 35457143
pii: ijms23084326
doi: 10.3390/ijms23084326
pmc: PMC9026418
pii:
doi:
Substances chimiques
Bacterial Proteins
0
Mucins
0
N-Acetylneuraminic Acid
GZP2782OP0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Swedish Research Council Formas
ID : 2018-01419
Organisme : Swedish Research Council
ID : 2016-05154
Organisme : Wilhelm and Martina Lundgren Foundation
ID : 2015-0699
Organisme : Engkvists Foundation
ID : 2015/108
Références
J Bacteriol. 1979 Jul;139(1):132-6
pubmed: 457600
J Appl Microbiol. 2018 Jun;124(6):1469-1479
pubmed: 29430777
J Bacteriol. 2004 May;186(10):3124-32
pubmed: 15126474
Infect Immun. 2017 Jul 19;85(8):
pubmed: 28533470
mBio. 2015 Jul 07;6(4):e00379
pubmed: 26152582
Infect Immun. 2014 Dec;82(12):5235-45
pubmed: 25287918
J Biol Chem. 2006 Aug 25;281(34):24398-404
pubmed: 16807235
Infect Immun. 2020 Apr 20;88(5):
pubmed: 32071069
Trends Cell Biol. 2004 Nov;14(11):648-56
pubmed: 15519854
Microorganisms. 2018 Jun 18;6(2):
pubmed: 29912166
BMC Genomics. 2008 Sep 18;9:427
pubmed: 18801193
Proc Natl Acad Sci U S A. 2008 Jul 15;105(28):9769-74
pubmed: 18606988
Sci Rep. 2017 Jan 20;7:40656
pubmed: 28106125
Fish Shellfish Immunol. 2022 Mar;122:181-190
pubmed: 35077869
PLoS Pathog. 2009 Oct;5(10):e1000617
pubmed: 19816567
Mol Microbiol. 2001 Oct;42(1):145-57
pubmed: 11679074
Anal Bioanal Chem. 2007 Jan;387(2):489-96
pubmed: 17143597
Microb Pathog. 2008 Nov-Dec;45(5-6):343-54
pubmed: 18930130
J Bacteriol. 1997 Sep;179(17):5271-81
pubmed: 9286976
Trends Microbiol. 2006 Mar;14(3):114-9
pubmed: 16459080
Bioorg Med Chem. 2008 Feb 15;16(4):1596-604
pubmed: 18053731
J Proteome Res. 2015 Aug 7;14(8):3239-51
pubmed: 26066491
FEMS Microbiol Lett. 1992 Nov 15;78(1):65-71
pubmed: 1468618
J Bacteriol. 1978 Aug;135(2):402-7
pubmed: 681278
Dis Aquat Organ. 2010 Feb 17;88(3):199-206
pubmed: 20377009
Mol Microbiol. 1994 Jul;13(2):273-86
pubmed: 7984107
Infect Immun. 2008 Apr;76(4):1445-55
pubmed: 18212071
Pathog Dis. 2015 Nov;73(8):ftv072
pubmed: 26377182
Dis Aquat Organ. 2005 Jun 14;65(1):43-52
pubmed: 16042042
Mol Cell. 2004 Sep 10;15(5):677-87
pubmed: 15350213
Curr Opin Microbiol. 2003 Apr;6(2):191-7
pubmed: 12732311
Infect Immun. 2003 Jul;71(7):3914-9
pubmed: 12819077
J Bacteriol. 2006 Jun;188(11):3952-61
pubmed: 16707687
Fish Shellfish Immunol. 2017 May;64:260-269
pubmed: 28279794
Mucosal Immunol. 2008 May;1(3):183-97
pubmed: 19079178
Cell Host Microbe. 2016 Apr 13;19(4):470-80
pubmed: 26996306
J Microbiol Methods. 2006 Sep;66(3):497-503
pubmed: 16564586
Nat Rev Microbiol. 2005 May;3(5):383-96
pubmed: 15864263
Appl Environ Microbiol. 1993 Aug;59(8):2411-7
pubmed: 8368830
Dis Aquat Organ. 2002 Aug 15;51(1):13-25
pubmed: 12240967
J Fish Dis. 2008 Feb;31(2):141-51
pubmed: 18234022
J Proteome Res. 2019 Apr 5;18(4):1760-1773
pubmed: 30848132
J Bacteriol. 1997 Sep;179(17):5292-9
pubmed: 9286979
J Bacteriol. 1999 Mar;181(5):1623-9
pubmed: 10049396
Acta Pathol Microbiol Scand B. 1981 Dec;89(6):387-97
pubmed: 7336925