Investigations on SARS-CoV-2 Susceptibility of Domestic and Wild Animals Using Primary Cell Culture Models Derived from the Upper and Lower Respiratory Tract.

ACE2 CTSL SARS-CoV-2 TMPRSS2 air–liquid interface animals primary cell cultures respiratory tract tissue explants zoonosis

Journal

Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722

Informations de publication

Date de publication:
16 04 2022
Historique:
received: 07 03 2022
revised: 14 04 2022
accepted: 14 04 2022
entrez: 23 4 2022
pubmed: 24 4 2022
medline: 27 4 2022
Statut: epublish

Résumé

Several animal species are susceptible to SARS-CoV-2 infection, as documented by case reports and serological and in vivo infection studies. However, the susceptibility of many animal species remains unknown. Furthermore, the expression patterns of SARS-CoV-2 entry factors, such as the receptor angiotensin-converting enzyme 2 (ACE2), as well as transmembrane protease serine subtype 2 (TMPRSS2) and cathepsin L (CTSL), cellular proteases involved in SARS-CoV-2 spike protein activation, are largely unexplored in most species. Here, we generated primary cell cultures from the respiratory tract of domestic and wildlife animals to assess their susceptibility to SARS-CoV-2 infection. Additionally, the presence of

Identifiants

pubmed: 35458558
pii: v14040828
doi: 10.3390/v14040828
pmc: PMC9032458
pii:
doi:

Substances chimiques

Spike Glycoprotein, Coronavirus 0
spike protein, SARS-CoV-2 0
Angiotensin-Converting Enzyme 2 EC 3.4.17.23

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

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Auteurs

Iris Färber (I)

Department of Pathology, University of Veterinary Medicine, Foundation, 30559 Hannover, Germany.

Johannes Krüger (J)

Department of Pathology, University of Veterinary Medicine, Foundation, 30559 Hannover, Germany.

Cheila Rocha (C)

Infection Biology Unit, German Primate Center, Leibniz Institute for Primate Research, 37077 Göttingen, Germany.

Federico Armando (F)

Department of Pathology, University of Veterinary Medicine, Foundation, 30559 Hannover, Germany.

Maren von Köckritz-Blickwede (M)

Department of Biochemistry, University of Veterinary Medicine Hannover, Foundation, 30559 Hannover, Germany.
Research Center for Emerging Infections and Zoonoses, University of Veterinary Medicine, Foundation, 30559 Hannover, Germany.

Stefan Pöhlmann (S)

Infection Biology Unit, German Primate Center, Leibniz Institute for Primate Research, 37077 Göttingen, Germany.
Faculty of Biology and Psychology, Georg-August-University, 37073 Göttingen, Germany.

Armin Braun (A)

Fraunhofer Institute for Toxicology and Experimental Medicine ITEM, 30625 Hannover, Germany.

Wolfgang Baumgärtner (W)

Department of Pathology, University of Veterinary Medicine, Foundation, 30559 Hannover, Germany.

Sandra Runft (S)

Department of Pathology, University of Veterinary Medicine, Foundation, 30559 Hannover, Germany.

Nadine Krüger (N)

Infection Biology Unit, German Primate Center, Leibniz Institute for Primate Research, 37077 Göttingen, Germany.

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