Detection of muscle stem cell-derived myonuclei in murine overloaded muscles.

Animals Cell Nucleus Mice Muscle, Skeletal Satellite Cells, Skeletal Muscle
Cell Biology Microscopy Stem Cells

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
17 06 2022
Historique:
entrez: 25 4 2022
pubmed: 26 4 2022
medline: 27 4 2022
Statut: epublish

Résumé

Muscle satellite cells (MuSCs) supply nuclei to existing myofibers in response to mechanical loading. This myonuclear accretion is critical for efficient muscle hypertrophy. Herein, we present protocols for the detection of MuSC-derived new myonuclei in loaded mouse muscle, including procedures for EdU injection to stain myonuclei, followed by surgery and skeletal muscle fixation. We then describe immunostaining for EdU

Identifiants

DOI: 10.1016/j.xpro.2022.101307 PMID: 35463471 PMC: PMC9018451
pubmed: 35463471
doi: 10.1016/j.xpro.2022.101307
pii: S2666-1667(22)00187-3
pmc: PMC9018451
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

101307

Informations de copyright

© 2022 The Author(s).

Déclaration de conflit d'intérêts

The authors declare no competing financial interests.

Références

Cell Stem Cell. 2022 Feb 3;29(2):265-280.e6
pubmed: 34856120
Nature. 2009 Jul 30;460(7255):627-31
pubmed: 19554048
FASEB J. 2014 Apr;28(4):1654-65
pubmed: 24376025
Skelet Muscle. 2017 Jul 10;7(1):14
pubmed: 28693603
Elife. 2019 Sep 23;8:
pubmed: 31545169
Biochim Biophys Acta Mol Cell Res. 2020 Sep;1867(9):118742
pubmed: 32417255
Development. 2011 Sep;138(17):3657-66
pubmed: 21828094

Auteurs

Akihiro Kaneshige (A)

Project for Muscle Stem Cell Biology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan.
Biological/Pharmacological Research Laboratories, Central Pharmaceutical Research Institute, Japan Tobacco Inc., 1-1 Murasaki-cho, Takatsuki, Osaka 569-1125, Japan.
Laboratory of Molecular and Cellular Physiology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan.

Takayuki Kaji (T)

Project for Muscle Stem Cell Biology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan.

Hayato Saito (H)

Project for Muscle Stem Cell Biology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan.

Tatsuyoshi Higashimoto (T)

Project for Muscle Stem Cell Biology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan.

Ayasa Nakamura (A)

Project for Muscle Stem Cell Biology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan.

Tamaki Kurosawa (T)

Department of Nutritional Physiology, Institute of Medical Nutrition, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan.
Laboratory of Veterinary Pharmacology, Department of Veterinary Medical Sciences, Graduate School of Agriculture and Life Sciences, Tokyo University, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

Madoka Ikemoto-Uezumi (M)

Department of Nutritional Physiology, Institute of Medical Nutrition, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan.

Akiyoshi Uezumi (A)

Department of Nutritional Physiology, Institute of Medical Nutrition, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan.

So-Ichiro Fukada (SI)

Project for Muscle Stem Cell Biology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Detection of muscle stem cell-derived...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Noyau de la cellule › Detection of muscle stem cell-derived...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Detection of muscle stem cell-derived...
questionsmedicales.fr › Tissus › Muscles › Muscle strié › Muscles squelettiques › Detection of muscle stem cell-derived...
questionsmedicales.fr › Cellules › Cellules souches › Myoblastes › Myoblastes squelettiques › Cellules satellites du muscle squelettique › Detection of muscle stem cell-derived...