Physiological Analysis of Phototropic Responses to Blue and Red Light in Arabidopsis.
Arabidopsis
Phototropin
Phototropism
Phytochrome
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
25
4
2022
pubmed:
26
4
2022
medline:
28
4
2022
Statut:
ppublish
Résumé
Plants utilize light as sole energy source. To maximize light capture, they are able to detect the light direction and orient themselves toward the light source. This phototropic response is mediated by the plant blue-light photoreceptors phototropin1 and phototropin2 (phot1 and phot2). Although fully differentiated plants also exhibit this response, it can be best observed in etiolated seedlings. Differences in light between the illuminated and shaded site of a seedling stem lead to changes in the auxin distribution, resulting in cell elongation on the shaded site. Since phototropism connects light perception, signaling, and auxin transport, it is of great interest to analyze this response with a fast and simple method. Moreover, pre-exposure to red light enhances the phototropic response via phytochrome A (phyA) and phyB action. Here we describe a method to analyze the phototropic response of Arabidopsis seedlings to blue light and the enhanced response with a red-light pretreatment. With numerous mutants available, its fast germination, and its small size, Arabidopsis is well suited for this analysis. Different genotypes can be simultaneously probed in less than a week.
Identifiants
pubmed: 35467199
doi: 10.1007/978-1-0716-2297-1_4
doi:
Substances chimiques
Arabidopsis Proteins
0
Indoleacetic Acids
0
Photoreceptors, Plant
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
37-45Informations de copyright
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
Whippo CW, Hangarter RP (2006) Phototropism: bending towards enlightenment. Plant Cell 18:1110–1119
doi: 10.1105/tpc.105.039669
Darwin C (1880) The power of movements in plants. John Murray, London
doi: 10.5962/bhl.title.102319
Went FW (1926) On growth accelerating substances in the coleoptile of Avena sativa. Proc K Akad Wet 30:10–19
Cholodny N (1927) Wuchshormone und Tropismen bei den Pflanzen. Biol Zentralbl 47:604–626
Kögel F, Haagen-Smit AJ (1931) Mitteilung über pflanzliche Wachstumsstoffe. Über die Chemie des Wuchsstoffes. Proc K Ned Akad Wet 34:1411–1416
Huala E, Oeller PW, Liscum E, Han IS, Larsen E, Briggs WR (1997) Arabidopsis NPH1: a protein kinase with a putative redox-sensing domain. Science 278:2120–2123
doi: 10.1126/science.278.5346.2120
Sakai T, Kagawa T, Kasahara M, Swartz TE, Christie JM, Briggs WR, Wada M, Okada K (2001) Arabidopsis nph1 and npl1: blue light receptors that mediate both phototropism and chloroplast relocation. Proc Natl Acad Sci U S A 98:6969–6974
doi: 10.1073/pnas.101137598
Kagawa T, Kimura M, Wada M (2009) Blue light-induced phototropism of inflorescence stems and petioles is mediated by phototropin family members phot1 and phot2. Plant Cell Physiol 50:1774–1785
doi: 10.1093/pcp/pcp119
Liscum E, Askinosie SK, Leuchtman DL, Morrow J, Willenburg KT, Coats DR (2014) Phototropism: growing towards an understanding of plant movement. Plant Cell 26:38–55
doi: 10.1105/tpc.113.119727
Fankhauser C, Christie JM (2015) Plant phototropic growth. Curr Biol 25:384–389
doi: 10.1016/j.cub.2015.03.020
Okajima K, Matsuoka D, Tokutomi S (2011) LOV2-linker-kinase phosphorylates LOV1-containing N-terminal polypeptide substrate via photoreaction of LOV2 in Arabidopsis phototropin1. FEBS Lett 585:3391–3395
doi: 10.1016/j.febslet.2011.10.003
Kagawa T, Sakai T, Suetsugu N, Oikawa K, Ishiguro S, Kato T, Tabata S, Okada K, Wada M (2001) Arabidopsis NPL1: a phototropin homolog controlling the chloroplast high-light avoidance response. Science 291:2138–2141
doi: 10.1126/science.291.5511.2138
Vandenbussche F, Tilbrook K, Fierro AC, Marchal K, Poelman D, Van Der Straeten D, Ulm R (2014) Photoreceptor-mediated bending towards UV-B in Arabidopsis. Mol Plant 7:1041–1052
doi: 10.1093/mp/ssu039
Vanhaelewyn L, Viczián A, Prinsen E, Bernula P, Serrano AM, Arana MV, Ballaré CL, Nagy F, Van Der Straeten D, Vandenbussche F (2019) Differential UVR8 signal across the stem controls UV-B-induced inflorescence phototropism. Plant Cell 31:2070–2088
doi: 10.1105/tpc.18.00929
Ahmad M, Jarillo JA, Smirnova O, Cashmore AR (1998) Cryptochrome blue-light photoreceptors of Arabidopsis implicated in phototropism. Nature 392:720–723
doi: 10.1038/33701
Parks BM, Quail PH, Hangarter RP (1996) Phytochrome A regulates red-light induction of phototropic enhancement in Arabidopsis. Plant Physiology 110:155–162
doi: 10.1104/pp.110.1.155
Lariguet P, Fankhauser C (2004) Hypocotyl growth orientation in blue light is determined by phytochrome A inhibition of gravitropism and phototropin promotion of phototropism. Plant J 40:826–834
doi: 10.1111/j.1365-313X.2004.02256.x
Rösler J, Klein I, Zeidler M (2007) Arabidopsis fhl/fhy1 double mutant reveals a distinct cytoplasmic action of phytochrome A. Proc Natl Acad Sci U S A 104:10737–10742
doi: 10.1073/pnas.0703855104
Rösler J, Jaedicke K, Zeidler M (2010) Cytoplasmic phytochrome action. Plant Cell Physiol 51:1248–1254
doi: 10.1093/pcp/pcq091
Jaedicke K, Lichtenthaler AL, Meyberg R, Zeidler M, Hughes J (2012) A phytochrome-phototropin light signaling complex at the plasma membrane. Proc Natl Acad Sci U S A 109:12231–12236
doi: 10.1073/pnas.1120203109
Ohgishi M, Saji K, Okada K, Sakai T (2004) Functional analysis of each blue light receptor, cry1, cry2, phot1, and phot2, by using combinatorial multiple mutants in Arabidopsis. Proc Natl Acad Sci U S A 101:2223–2228
doi: 10.1073/pnas.0305984101
Hangarter RP (1997) Gravity, light and plant form. Plant Cell Environ 20:796–800
doi: 10.1046/j.1365-3040.1997.d01-124.x
Janoudi AK, Gordon WR, Wagner D, Quail P, Poff KL (1997) Multiple phytochromes are involved in red-light-induced enhancement of first-positive phototropism in Arabidopsis thaliana. Plant Physiol 113:975–979
doi: 10.1104/pp.113.3.975
Tsuchida-Mayama T, Sakai T, Hanada A, Uehara Y, Asami T, Yamaguchi S (2010) Role of the phytochrome and cryptochrome signaling pathways in hypocotyl phototropism. Plant J 62:653–662
doi: 10.1111/j.1365-313X.2010.04180.x
Motchoulski A, Liscum E (1999) Arabidopsis NPH3: a NPH1 photoreceptor-interacting protein essential for phototropism. Science 286:961–964
doi: 10.1126/science.286.5441.961
Sakai T, Wada T, Ishiguro S, Okada K (2000) RPT2. A signal transducer of the phototropic response in Arabidopsis. Plant Cell 12:225–236
doi: 10.1105/tpc.12.2.225
Lariguet P, Schepens I, Hodgson D, Pedmale UV, Trevisan M, Kami C, de Carbonnel M, Alonso JM, Ecker JR, Liscum E, Fankhauser C (2006) Phytochrome kinase substrate 1 is a phototropin 1 binding protein required for phototropism. Proc Natl Acad Sci U S A 103:10134–10139
doi: 10.1073/pnas.0603799103
de Carbonnel M, Davis P, Roelfsema MR, Inoue S, Schepens I, Lariguet P, Geisler M, Shimazaki K, Hangarter R, Fankhauser C (2010) The Arabidopsis phytochrome kinase substrate 2 protein is a phototropin signaling element that regulates leaf flattening and leaf positioning. Plant Physiol 152:1391–1405
doi: 10.1104/pp.109.150441
Demarsy E, Schepens I, Okajima K, Hersch M, Bergmann S, Christie J, Shimazaki K, Tokutomi S, Fankhauser C (2012) Phytochrome kinase substrate 4 is phosphorylated by the phototropin 1 photoreceptor. EMBO J 31:3457–3467
doi: 10.1038/emboj.2012.186
Haga K, Takano M, Neumann R, Iino M (2005) The rice coleoptile phototropism 1 gene encoding an ortholog of Arabidopsis NPH3 is required for phototropism of coleoptiles and lateral translocation of auxin. Plant Cell 17:103–115
doi: 10.1105/tpc.104.028357
Kami C, Allenbach L, Zourelidou M, Ljung K, Schutz F, Isono E, Watahiki MK, Yamamoto KT, Schwechheimer C, Fankhauser C (2014) Reduced phototropism in pks mutants may be due to altered auxin-regulated gene expression or reduced lateral auxin transport. Plant J 77:393–403
doi: 10.1111/tpj.12395
Friml J, Wisniewska J, Benkova E, Mendgen K, Palme K (2002) Lateral relocation of auxin efflux regulator PIN3 mediates tropism in Arabidopsis. Nature 415:806–809
doi: 10.1038/415806a
Ding Z, Galvan-Ampudia CS, Demarsy E, Langowski L, Kleine-Vehn J, Fan Y, Morita MT, Tasaka M, Fankhauser C, Offringa R, Friml J (2011) Light-mediated polarization of the PIN3 auxin transporter for the phototropic response in Arabidopsis. Nature Cell Biol 13:447–452
doi: 10.1038/ncb2208
Noh B, Murphy AS, Spalding EP (2001) Multidrug resistance-like genes of Arabidopsis required for auxin transport and auxin-mediated development. Plant Cell 13:2441–2454
pubmed: 11701880
pmcid: 139463
Nagashima A, Uehara Y, Sakai T (2008) The ABC subfamily B auxin transporter AtABCB19 is involved in the inhibitory effects of N-1-naphthyphthalamic acid on the phototropic and gravitropic responses of Arabidopsis hypocotyls. Plant Cell Physiol 49:1250–1255
doi: 10.1093/pcp/pcn092
Christie JM, Yang H, Richter GL, Sullivan S, Thomson CE, Lin J, Titapiwatanakun B, Ennis M, Kaiserli E, Lee OR, Adamec J, Peer WA, Murphy AS (2011) phot1 inhibition of ABCB19 primes lateral auxin fluxes in the shoot apex required for phototropism. PLoS Biol 9:e1001076
doi: 10.1371/journal.pbio.1001076
Stone BB, Stowe-Evans EL, Harper RM, Celaya RB, Ljung K, Sandberg G, Liscum E (2008) Disruptions in AUX1-dependent auxin influx alter hypocotyl phototropism in Arabidopsis. Mol Plant 1:129–144
doi: 10.1093/mp/ssm013
Whippo CW, Hangarter RP (2003) Second positive phototropism results from coordinated co-action of the phototropins and cryptochromes. Plant Physiol 132:1499
doi: 10.1104/pp.102.018481
Yamamoto K, Suzuki T, Aihara Y, Haga K, Sakai T, Nagatani A (2014) The phototropic response is locally regulated within the topmost light-responsive region of the Arabidopsis thaliana seedling. Plant Cell Physiol 55:497–506
doi: 10.1093/pcp/pct184