Unveiling molecular interactions that stabilize bacterial adhesion pili.
Journal
Biophysical journal
ISSN: 1542-0086
Titre abrégé: Biophys J
Pays: United States
ID NLM: 0370626
Informations de publication
Date de publication:
07 06 2022
07 06 2022
Historique:
received:
20
01
2022
revised:
09
03
2022
accepted:
27
04
2022
pubmed:
3
5
2022
medline:
11
6
2022
entrez:
2
5
2022
Statut:
ppublish
Résumé
Adhesion pili assembled by the chaperone-usher pathway are superelastic helical filaments on the surface of bacteria, optimized for attachment to target cells. Here, we investigate the biophysical function and structural interactions that stabilize P pili from uropathogenic bacteria. Using optical tweezers, we measure P pilus subunit-subunit interaction dynamics and show that pilus compliance is contour-length dependent. Atomic details of subunit-subunit interactions of pili under tension are shown using steered molecular dynamics (sMD) simulations. sMD results also indicate that the N-terminal "staple" region of P pili, which provides interactions with pilins that are four and five subunits away, significantly stabilizes the helical filament structure. These data are consistent with previous structural data, and suggest that more layer-to-layer interactions could compensate for the lack of a staple in type 1 pili. This study informs our understanding of essential structural and dynamic features of adhesion pili, supporting the hypothesis that the function of pili is critically dependent on their structure and biophysical properties.
Identifiants
pubmed: 35491503
pii: S0006-3495(22)00364-2
doi: 10.1016/j.bpj.2022.04.036
pmc: PMC9247471
pii:
doi:
Substances chimiques
Escherichia coli Proteins
0
Molecular Chaperones
0
Fimbriae Proteins
147680-16-8
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2096-2106Informations de copyright
Copyright © 2022 Biophysical Society. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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