Calcium acts as a central player in melatonin antitumor activity in sarcoma cells.
Calcium
Chondrosarcoma
Melatonin
Migration
Osteosarcoma
Proliferation
Journal
Cellular oncology (Dordrecht)
ISSN: 2211-3436
Titre abrégé: Cell Oncol (Dordr)
Pays: Netherlands
ID NLM: 101552938
Informations de publication
Date de publication:
Jun 2022
Jun 2022
Historique:
accepted:
08
04
2022
pubmed:
3
5
2022
medline:
15
6
2022
entrez:
2
5
2022
Statut:
ppublish
Résumé
Chondrosarcoma and osteosarcoma are the most frequently occurring bone cancers. Although surgery and chemotherapy are currently clinically applied, improved treatment options are urgently needed. Melatonin is known to inhibit cell proliferation in both tumor types. Although the underlying mechanisms are not clear yet, calcium homeostasis has been reported to be a key factor in cancer biology. Here, we set out to investigate whether regulation of calcium by this indolamine may be involved in its antitumor effect. Cell viability was measured using a MTT assay and flow cytometry was used to measure levels of cytosolic calcium, intracellular oxidants, mitochondrial membrane potential and cell cycle progression. Mitochondrial calcium was analyzed by fluorimetry. Cell migration was determined using a scratch wound-healing assay. Western blot analysis was used to assess the expression of proteins related to cell cycle progression, epithelial to mesenchymal transition (EMT), Ac-CoA synthesis and intracellular signaling pathways. We found that melatonin decreases cytosolic and mitochondrial Ca Our data indicate that regulation of calcium homeostasis is a key factor in the inhibition of cell proliferation and migration by melatonin. This effect should be taken into consideration in combined therapies with traditional or new antitumor compounds, since it may circumvent therapy resistance.
Identifiants
pubmed: 35499815
doi: 10.1007/s13402-022-00674-9
pii: 10.1007/s13402-022-00674-9
pmc: PMC9187547
doi:
Substances chimiques
Melatonin
JL5DK93RCL
Calcium
SY7Q814VUP
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
415-428Informations de copyright
© 2022. The Author(s).
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