A short-term chick embryo


Journal

Indian journal of ophthalmology
ISSN: 1998-3689
Titre abrégé: Indian J Ophthalmol
Pays: India
ID NLM: 0405376

Informations de publication

Date de publication:
05 2022
Historique:
entrez: 3 5 2022
pubmed: 4 5 2022
medline: 6 5 2022
Statut: ppublish

Résumé

Cancer stem cells (CSCs) reported in various tumors play a crucial role in tumorigenesis and metastasis of retinoblastoma (Rb). Following the efforts to reduce, replace, and refine the use of mammalian models, we aimed to establish a short-term xenograft for Rb to evaluate the CSC properties of CD133 Y79 cells were cultured, labeled with two different dyes (CM-Dil Y79 and enhanced green fluorescent protein (eGFP)) and sorted for CD133 Y79 cells formed pink-white raised perivascular nodules with feeder vessels on the CAM with both the types of labeled CD133 This study highlights that CE-CAM is a feasible alternative nonmammalian model for evaluating tumorigenicity and metastatic potential of Y79 CSCs. Increased tumorigenicity and metastatic potential of CD133

Identifiants

pubmed: 35502056
pii: IndianJOphthalmol_2022_70_5_1703_343968
doi: 10.4103/ijo.IJO_2348_21
pmc: PMC9332960
doi:

Substances chimiques

AC133 Antigen 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1703-1711

Commentaires et corrections

Type : CommentIn

Déclaration de conflit d'intérêts

None

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Auteurs

Rohini M Nair (RM)

School of Medical Sciences, University of Hyderabad, Hyderabad, India; Department of Ophthalmology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States.

Narayana V L Revu (NVL)

School of Medical Sciences, University of Hyderabad, Hyderabad, India.

Sucharita Gali (S)

School of Medical Sciences, University of Hyderabad, Hyderabad, India.

Prathap Reddy Kallamadi (PR)

Animal Facility, National Institute of Nutrition, Hyderabad, India.

Varsha Prabhu (V)

School of Medical Sciences, University of Hyderabad, Hyderabad, India.

Radhika Manukonda (R)

School of Medical Sciences, University of Hyderabad, Hyderabad, India.

Harishankar Nemani (H)

Animal Facility, National Institute of Nutrition, Hyderabad, India.

Swathi Kaliki (S)

The Operation Eyesight Universal Institute for Eye Cancer; Ophthalmic Pathology Laboratory, L V Prasad Eye Institute, Hyderabad, India.

Geeta K Vemuganti (GK)

School of Medical Sciences, University of Hyderabad, Hyderabad, India.

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Classifications MeSH