Construction of nano receptors for ubiquitin and ubiquitinated proteins based on the region-specific interactions between ubiquitin and polydopamine.

Antibodies, Monoclonal Dopamine Indoles Molecular Imprinting / methods Molecularly Imprinted Polymers Polymers Receptors, Artificial Ubiquitin Ubiquitinated Proteins / chemistry

Journal

Journal of materials chemistry. B
ISSN: 2050-7518
Titre abrégé: J Mater Chem B
Pays: England
ID NLM: 101598493

Informations de publication

Date de publication:
15 09 2022
Historique:
pubmed: 5 5 2022
medline: 20 9 2022
entrez: 4 5 2022
Statut: epublish

Résumé

Ubiquitination is a prevalent post-translational modification that controls a multitude of important biological processes. Due to the low abundance of ubiquitinated proteins, highly efficient separation and enrichment approaches are required for ubiquitinome analysis. In this work, we disclose the region-specific interactions between the hydrophobic patch of ubiquitin and polydopamine. Taking advantage of this inherent binding property, we have constructed surface-imprinted magnetic nanoparticles (NPs) for ubiquitin by sequential dopamine polymerization and surface PEGylation. The obtained molecularly imprinted polymer (MIP) NPs showed a binding constant of 2.6 × 10

Identifiants

DOI: 10.1039/d2tb00255h PMID: 35506347
pubmed: 35506347
doi: 10.1039/d2tb00255h
doi:

Substances chimiques

Antibodies, Monoclonal 0
Indoles 0
Molecularly Imprinted Polymers 0
Polymers 0
Receptors, Artificial 0
Ubiquitin 0
Ubiquitinated Proteins 0
polydopamine 0
Dopamine VTD58H1Z2X

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

6627-6633

Auteurs

Zezhou Li (Z)

Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. mpzhao@pku.edu.cn.

Xinyi Li (X)

Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. mpzhao@pku.edu.cn.

Wei Xian (W)

Department of Microbiology and Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China. xiaoyun.liu@bjmu.edu.cn.

Huaisyuan Xie (H)

Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. mpzhao@pku.edu.cn.

Ying Sun (Y)

Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. mpzhao@pku.edu.cn.

Yuxuan Zhang (Y)

Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. mpzhao@pku.edu.cn.

Jiayu Wang (J)

Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. mpzhao@pku.edu.cn.

Hongwei Li (H)

Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. mpzhao@pku.edu.cn.

Changwen Jin (C)

Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. mpzhao@pku.edu.cn.

Xiaoyun Liu (X)

Department of Microbiology and Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China. xiaoyun.liu@bjmu.edu.cn.

Zhiwei Zhu (Z)

Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. mpzhao@pku.edu.cn.
ORCID: 0000-0001-8119-4166

Meiping Zhao (M)

Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. mpzhao@pku.edu.cn.
ORCID: 0000-0002-8696-8738

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Anticorps monoclonaux › Construction of nano receptors for ubiquitin...
questionsmedicales.fr › Composés chimiques organiques › Hydrocarbures › Hydrocarbures cycliques › Hydrocarbures aromatiques › Dérivés du benzène › Phénols › Catéchols › Catécholamines › Dopamine › Construction of nano receptors for ubiquitin...
questionsmedicales.fr › Composés hétérocycliques › Composés hétérocycliques à cycles fusionnés › Composés hétérobicycliques › Indoles › Construction of nano receptors for ubiquitin...
questionsmedicales.fr › Technologie, industrie et agriculture › Technologie › Technologie pharmaceutique › Techniques de chimie synthétique › Empreinte moléculaire › Construction of nano receptors for ubiquitin...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Polymères › Polymères à empreintes moléculaires › Construction of nano receptors for ubiquitin...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Polymères › Construction of nano receptors for ubiquitin...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomimétiques › Récepteurs artificiels › Construction of nano receptors for ubiquitin...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Ubiquitines › Ubiquitine › Construction of nano receptors for ubiquitin...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines ubiquitinées › Construction of nano receptors for ubiquitin...