Influence of acidic pH on antimicrobial activity of different calcium silicate based-endodontic sealers.
Acidic pH
Antimicrobial properties
Calcium silicate–based sealer
Confocal laser scanning microscopy
Dentin
Journal
Clinical oral investigations
ISSN: 1436-3771
Titre abrégé: Clin Oral Investig
Pays: Germany
ID NLM: 9707115
Informations de publication
Date de publication:
Aug 2022
Aug 2022
Historique:
received:
18
11
2021
accepted:
14
04
2022
pubmed:
6
5
2022
medline:
19
8
2022
entrez:
5
5
2022
Statut:
ppublish
Résumé
To investigate the antibacterial activity of calcium silicate-based sealers (CSBSs) against Enterococcus faecalis biofilm in a neutral or acidic condition. Dentin cylinders (4 mm length) were prepared and infected with 3-week-old E. faecalis. The samples were filled with BioRoot RCS (BR), EndoSequence BC (ES), and NeoMTA Plus (NMTA) and incubated in either neutral or acidic conditions for 7 days (n=10/group). Sterile or infected samples alone were used as the positive and negative control. The root canal sealers were removed after 7 days, and the remaining bacteria on dentinal walls were determined by colony-forming units (CFUs/ml), and three samples from each group were visualized under a confocal laser scanning microscope (CLSM). The pH was also measured (n=3/group) after 4 h and 7 days of incubation at 37°C in both conditions. In the neutral condition, all sealers significantly decreased the log-CFU values (p<0.05), while in the acidic condition, the log-CFU reduction was less for ES and NMTA, but a higher reduction was observed in BR (p<0.05). The antibacterial activity of CSBSs was similar in neutral conditions (p>0.05), and BR showed a greater antibacterial effect than ES and NMTA in the acidic condition (p<0.05). The pH of BR, ES, and NMTA ranged from 8.2 to 8.8 in the neutral condition in the presence of dentin after 7 days. However, acidic conditions reduced the pH values to 7.8 for BR, 6.0 for ES, and 5.8 for NMTA. All CSBSs showed similar antibacterial activity in neutral conditions, while acidic pH had a reducing antibacterial effect on CSBSs. Inflammatory pH decreased the antibacterial properties of CSBSs depending on the sealer type.
Identifiants
pubmed: 35511289
doi: 10.1007/s00784-022-04504-y
pii: 10.1007/s00784-022-04504-y
doi:
Substances chimiques
Anti-Bacterial Agents
0
Calcium Compounds
0
Epoxy Resins
0
Root Canal Filling Materials
0
Silicates
0
calcium silicate
S4255P4G5M
Types de publication
Journal Article
Langues
eng
Pagination
5369-5376Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
Références
Nair PN, Henry S, Cano V, Vera J (2005) Microbial status of apical root canal system of human mandibular first molars with primary apical periodontitis after “one-visit” endodontic treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 99:231–52. https://doi.org/10.1016/j.tripleo.2004.10.005
doi: 10.1016/j.tripleo.2004.10.005
pubmed: 15660098
Zhang H, Shen Y, Ruse ND, Haapasalo M (2009) Antibacterial activity of endodontic sealers by modified direct contact test against Enterococcus faecalis. J Endod 35:1051–5. https://doi.org/10.1016/j.joen.2009.04.022
doi: 10.1016/j.joen.2009.04.022
pubmed: 19567333
Silva Almeida LH, Moraes RR, Morgental RD, Pappen FG (2017) Are premixed calcium silicate-based endodontic sealers comparable to conventional materials? A systematic review of in vitro studies. J Endod 43:527–535. https://doi.org/10.1016/j.joen.2016.11.019
doi: 10.1016/j.joen.2016.11.019
pubmed: 28216270
Alsubait S, Albader S, Alajlan N, Alkhunaini N, Niazy A, Almahdy A (2019) Comparison of the antibacterial activity of calcium silicate- and epoxy resin-based endodontic sealers against Enterococcus faecalis biofilms: a confocal laser-scanning microscopy analysis. Odontology 107:513–520. https://doi.org/10.1007/s10266-019-00425-7
doi: 10.1007/s10266-019-00425-7
pubmed: 30927150
Jeanneau C, Giraud T, Laurent P, About I (2019) BioRoot RCS extracts modulate the early mechanisms of periodontal inflammation and regeneration. J Endod 45:1016–1023. https://doi.org/10.1016/j.joen.2019.04.003
doi: 10.1016/j.joen.2019.04.003
pubmed: 31160081
Siboni F, Taddei P, Prati C, Gandolfi M (2017) Properties of NeoMTA Plus and MTA Plus cements for endodontics. Int Endod J 50:e83–e94. https://doi.org/10.1111/iej.12787
doi: 10.1111/iej.12787
pubmed: 28452115
Pinheiro LS, Iglesias JE, Boijink D, Mestieri LB, Poli Kopper PM, Figueiredo JAP, Grecca FS (2018) Cell viability and tissue reaction of NeoMTA Plus: an in vitro and in vivo study. J Endod 44:1140–1145. https://doi.org/10.1016/j.joen.2018.03.007
doi: 10.1016/j.joen.2018.03.007
pubmed: 29866406
Siboni F, Taddei P, Prati C, Gandolfi MG (2017) Properties of NeoMTA Plus and MTA Plus cements for endodontics. Int Endod J 50(Suppl 2):e83–e94. https://doi.org/10.1111/iej.12787
doi: 10.1111/iej.12787
pubmed: 28452115
Taha NA, Al-Khatib H (2022) 4-year follow-up of full pulpotomy in symptomatic mature permanent teeth with carious pulp exposure using a stainproof calcium silicate-based material. J Endod 48:87–95. https://doi.org/10.1016/j.joen.2021.09.008
doi: 10.1016/j.joen.2021.09.008
pubmed: 34563506
De-Deus G, Canabarro A (2017) Strength of recommendation for single-visit root canal treatment: grading the body of the evidence using a patient-centred approach. Int Endod J 50:251–259. https://doi.org/10.1111/iej.12621
doi: 10.1111/iej.12621
pubmed: 26878140
Paredes-Vieyra J, Enriquez FJJ (2012) Success rate of single- versus two-visit root canal treatment of teeth with apical periodontitis: a randomized controlled trial. J Endod 38:1164–1169. https://doi.org/10.1016/j.joen.2012.05.021
doi: 10.1016/j.joen.2012.05.021
pubmed: 22892729
Su Y, Wang C, Ye L (2011) Healing rate and post-obturation pain of single- versus multiple-visit endodontic treatment for infected root canals: a systematic review. J Endod 37:125–132. https://doi.org/10.1016/j.joen.2010.09.005
doi: 10.1016/j.joen.2010.09.005
pubmed: 21238790
Hojo S, Komatsu M, Okuda R, Takahashi N, Yamada T (1994) Acid profiles and pH of carious dentin in active and arrested lesions. J Dent Res 73:1853–7. https://doi.org/10.1177/00220345940730121001
doi: 10.1177/00220345940730121001
pubmed: 7814758
Baras BH, Sun J, Melo MAS, Tay FR, Oates TW, Zhang K, Weir MD, Xu HHK (2019) Novel root canal sealer with dimethylaminohexadecyl methacrylate, nano-silver and nano-calcium phosphate to kill bacteria inside root dentin and increase dentin hardness. Dent Mater 35:1479–1489. https://doi.org/10.1016/j.dental.2019.07.014
doi: 10.1016/j.dental.2019.07.014
pubmed: 31387742
Roy CO, Jeansonne BG, Gerrets TF (2001) Effect of an acid environment on leakage of root-end filling materials. J Endod 27:7–8. https://doi.org/10.1097/00004770-200101000-00002
doi: 10.1097/00004770-200101000-00002
pubmed: 11487169
Nassar M, Hiraishi N, Tamura Y, Otsuki M, Aoki K, Tagami J (2015) Phytic acid: an alternative root canal chelating agent. J Endod 41:242–247
doi: 10.1016/j.joen.2014.09.029
Neelakantan P, Berger T, Primus C, Shemesh H, Wesselink PR (2019) Acidic and alkaline chemicals’ influence on a tricalcium silicate-based dental biomaterial. J Biomed Mater Res B Appl Biomater 107:377–387. https://doi.org/10.1002/jbm.b.34129
doi: 10.1002/jbm.b.34129
pubmed: 29656513
Ashofteh Yazdi K, Ghabraei S, Bolhari B, Kafili M, Meraji N, Nekoofar MH, Dummer PMH (2019) Microstructure and chemical analysis of four calcium silicate-based cements in different environmental conditions. Clin Oral Investig 23:43–52. https://doi.org/10.1007/s00784-018-2394-1
doi: 10.1007/s00784-018-2394-1
pubmed: 29603021
Elnaghy AM (2014) Influence of acidic environment on properties of biodentine and white mineral trioxide aggregate: a comparative study. J Endod 40:953–957. https://doi.org/10.1016/j.joen.2013.11.007
doi: 10.1016/j.joen.2013.11.007
pubmed: 24935542
Wang Z, Ma J, Shen Y, Haapasalo M (2015) Acidic pH weakens the microhardness and microstructure of three tricalcium silicate materials. Int Endod J 48:323–332. https://doi.org/10.1111/iej.12318
doi: 10.1111/iej.12318
pubmed: 24871586
Azim AA, Aksel H, Zhuang T, Mashtare T, Babu JP, Huang GT-J (2016) Efficacy of 4 irrigation protocols in killing bacteria colonized in dentinal tubules examined by a novel confocal laser scanning microscope analysis. J Endod 42:928–934. https://doi.org/10.1016/j.joen.2016.03.009
doi: 10.1016/j.joen.2016.03.009
pubmed: 27130334
pmcid: 4884135
Bukhari S, Karabucak B (2019) The antimicrobial effect of bioceramic sealer on an 8-week matured Enterococcus faecalis biofilm attached to root canal dentinal surface. J Endod 45:1047–1052. https://doi.org/10.1016/j.joen.2019.04.004
doi: 10.1016/j.joen.2019.04.004
pubmed: 31160079
Urban K, Neuhaus J, Donnermeyer D, Schäfer E, Dammaschke T (2018) Solubility and pH value of 3 different root canal sealers: a long-term investigation. J Endod 44:1736–1740. https://doi.org/10.1016/j.joen.2018.07.026
doi: 10.1016/j.joen.2018.07.026
pubmed: 30243663
Wang Z, Shen Y, Haapasalo M (2014) Dentin extends the antibacterial effect of endodontic sealers against Enterococcus faecalis biofilms. J Endod 40:505–8. https://doi.org/10.1016/j.joen.2013.10.042
doi: 10.1016/j.joen.2013.10.042
pubmed: 24666900
Stuart CH, Schwartz SA, Beeson TJ, Owatz CB (2006) Enterococcus faecalis: its role in root canal treatment failure and current concepts in retreatment. J Endod 32:93–8. https://doi.org/10.1016/j.joen.2005.10.049
doi: 10.1016/j.joen.2005.10.049
pubmed: 16427453
Sedgley CM, Lee EH, Martin MJ, Flannagan SE (2008) Antibiotic resistance gene transfer between Streptococcus gordonii and Enterococcus faecalis in root canals of teeth ex vivo. J Endod 34:570–4. https://doi.org/10.1016/j.joen.2008.02.014
doi: 10.1016/j.joen.2008.02.014
pubmed: 18436036
Bronzato JD, Davidian MES, de Castro M, de-Jesus-Soares A, Ferraz CCR, Almeida JFA, Marciano MA, Gomes B (2021) Bacteria and virulence factors in periapical lesions associated with teeth following primary and secondary root canal treatment. Int Endod J 54:660–671 https://doi.org/10.1111/iej.13457
Kapralos V, Koutroulis A, Ørstavik D, Sunde PT, Rukke HV (2018) Antibacterial activity of endodontic sealers against planktonic bacteria and bacteria in biofilms. J Endod 44:149–154. https://doi.org/10.1016/j.joen.2017.08.023
doi: 10.1016/j.joen.2017.08.023
pubmed: 29153733
Faria-Júnior NB, Tanomaru-Filho M, Berbert FL, Guerreiro-Tanomaru JM (2013) Antibiofilm activity, pH and solubility of endodontic sealers. Int Endod J 46:755–62. https://doi.org/10.1111/iej.12055
doi: 10.1111/iej.12055
pubmed: 23441819
Candeiro GTM, Moura-Netto C, D’Almeida-Couto RS, Azambuja-Júnior N, Marques MM, Cai S, Gavini G (2016) Cytotoxicity, genotoxicity and antibacterial effectiveness of a bioceramic endodontic sealer. Int Endod J 49:858–864. https://doi.org/10.1111/iej.12523
doi: 10.1111/iej.12523
pubmed: 26281002
Tian J, Zhang Y, Lai Z, Li M, Huang Y, Jiang H, Wei X (2017) Ion release, microstructural, and biological properties of iRoot BP Plus and ProRoot MTA exposed to an acidic environment. J Endod 43:163–168. https://doi.org/10.1016/j.joen.2016.10.011
doi: 10.1016/j.joen.2016.10.011
pubmed: 27939732
Rodríguez-Lozano FJ, Collado-González M, López-García S, García-Bernal D, Moraleda JM, Lozano A, Forner L, Murcia L, Oñate-Sánchez RE (2019) Evaluation of changes in ion release and biological properties of NeoMTA-Plus and Endocem-MTA exposed to an acidic environment. Int Endod J 52:1196–1209. https://doi.org/10.1111/iej.13107
doi: 10.1111/iej.13107
pubmed: 30828816
Barros J, Silva MG, Rôças IN, Gonçalves LS, Alves FF, Lopes MA, Pina-Vaz I, Siqueira JF Jr (2014) Antibiofilm effects of endodontic sealers containing quaternary ammonium polyethylenimine nanoparticles. J Endod 40:1167–71. https://doi.org/10.1016/j.joen.2013.12.021
doi: 10.1016/j.joen.2013.12.021
pubmed: 25069926
Zhang W, Li Z, Peng B (2010) Ex vivo cytotoxicity of a new calcium silicate-based canal filling material. Int Endod J 43:769–74. https://doi.org/10.1111/j.1365-2591.2010.01733.x
doi: 10.1111/j.1365-2591.2010.01733.x
pubmed: 20546044
Farhad A, Mohammadi Z (2005) Calcium hydroxide: a review. Int Dent J 55:293–301. https://doi.org/10.1111/j.1875-595x.2005.tb00326.x
doi: 10.1111/j.1875-595x.2005.tb00326.x
pubmed: 16245464
Koutroulis A, Kuehne SA, Cooper PR, Camilleri J (2019) The role of calcium ion release on biocompatibility and antimicrobial properties of hydraulic cements. Sci Rep 9:19019. https://doi.org/10.1038/s41598-019-55288-3
doi: 10.1038/s41598-019-55288-3
pubmed: 31836731
pmcid: 6910940
Zehnder M, Waltimo T, Sener B, Soderling E (2006) Dentin enhances the effectiveness of bioactive glass S53P4 against a strain of Enterococcus faecalis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 101:530–5. https://doi.org/10.1016/j.tripleo.2005.03.014
doi: 10.1016/j.tripleo.2005.03.014
pubmed: 16545719
Nerwich AH, Figdor D, Messer HH (1993) pH changes in root dentine following calcium hydroxide intracanal dressing. Aust Endod Newsl 19:30–31. https://doi.org/10.1111/j.1747-4477.1993.tb00393.x
doi: 10.1111/j.1747-4477.1993.tb00393.x