Effect of solvent composition on the extraction of proteins from hemp oil processing stream.
alkali extraction
amino acid analysis
hemp proteins
plant-based proteins
salt extraction
Journal
Journal of the science of food and agriculture
ISSN: 1097-0010
Titre abrégé: J Sci Food Agric
Pays: England
ID NLM: 0376334
Informations de publication
Date de publication:
Nov 2022
Nov 2022
Historique:
revised:
29
04
2022
received:
04
11
2021
accepted:
05
05
2022
pubmed:
7
5
2022
medline:
5
10
2022
entrez:
6
5
2022
Statut:
ppublish
Résumé
Hempseed meal, a by-product of the hempseed oil processing stream, is a potential alternative source for food proteins. Efficient extraction of proteins from hempseed meal is challenging owing to differences in the structure and solubility of various protein fractions present in the seed. In the present study, protein was extracted from hempseed meal using four different solvents, including aqueous NaOH, KOH, NaHCO Extraction using alkaline solvents provided superior protein recovery (60-78%) compared with NaCl solution and control extractions (20-48% and 21%, respectively). The concentration of alkali or salt (0.25-1 mol L Although alkali solvents provide superior extraction yields, extraction with water resulted in extracts containing the highest proportion of proteins bearing essential amino acids. According to the results of SDS-PAGE, extraction using alkali solvents induced protein crosslinking. © 2022 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Sections du résumé
BACKGROUND
BACKGROUND
Hempseed meal, a by-product of the hempseed oil processing stream, is a potential alternative source for food proteins. Efficient extraction of proteins from hempseed meal is challenging owing to differences in the structure and solubility of various protein fractions present in the seed. In the present study, protein was extracted from hempseed meal using four different solvents, including aqueous NaOH, KOH, NaHCO
RESULTS
RESULTS
Extraction using alkaline solvents provided superior protein recovery (60-78%) compared with NaCl solution and control extractions (20-48% and 21%, respectively). The concentration of alkali or salt (0.25-1 mol L
CONCLUSION
CONCLUSIONS
Although alkali solvents provide superior extraction yields, extraction with water resulted in extracts containing the highest proportion of proteins bearing essential amino acids. According to the results of SDS-PAGE, extraction using alkali solvents induced protein crosslinking. © 2022 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Identifiants
pubmed: 35514139
doi: 10.1002/jsfa.11979
pmc: PMC9790205
doi:
Substances chimiques
Albumins
0
Amino Acids
0
Amino Acids, Essential
0
Plant Extracts
0
Solvents
0
Water
059QF0KO0R
Sodium Dodecyl Sulfate
368GB5141J
Sodium Chloride
451W47IQ8X
Sodium Hydroxide
55X04QC32I
hempseed oil
69VJ1LPN1S
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6293-6298Subventions
Organisme : Irish Department of Agriculture, Food and the Marine
ID : 2019PROG702
Organisme : Department of Agriculture
Informations de copyright
© 2022 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
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