Successive Site Translocating Inoculation Improved T Cell Responses Elicited by a DNA Vaccine Encoding SARS-CoV-2 S Protein.


Journal

Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960

Informations de publication

Date de publication:
2022
Historique:
received: 14 02 2022
accepted: 28 03 2022
entrez: 6 5 2022
pubmed: 7 5 2022
medline: 10 5 2022
Statut: epublish

Résumé

A variety of methods have been explored to increase delivery efficiencies for DNA vaccine. However, the immunogenicity of DNA vaccines has not been satisfactorily improved. Unlike most of the previous attempts, we provided evidence suggesting that changing the injection site successively (successively site-translocated inoculation, SSTI) could significantly enhance the immunogenicity of DNA vaccines in a previous study. To simplify the strategy and to evaluate its impact on candidate SARS-CoV-2 vaccines, we immunized mice with either a SARS-CoV-2 spike-based DNA vaccine or a spike protein subunit vaccine

Identifiants

pubmed: 35514964
doi: 10.3389/fimmu.2022.875236
pmc: PMC9062103
doi:

Substances chimiques

COVID-19 Vaccines 0
Interleukin-2 0
Protein Subunits 0
Spike Glycoprotein, Coronavirus 0
Tumor Necrosis Factor-alpha 0
Vaccines, DNA 0
spike protein, SARS-CoV-2 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

875236

Informations de copyright

Copyright © 2022 Tian, Zhang, He, Li, Yan, Zhu, Wan and Wang.

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Auteurs

Xiangxiang Tian (X)

Department of Medical Laboratory, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Department of Infectious Disease, Shanghai Key Laboratory of Infectious Diseases and Biosafety Emergency Response, National Medical Center for Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China.
Department of Laboratory Medicine, Shanghai Public Health Clinical Center, Shanghai, China.

Yifan Zhang (Y)

Department of Medical Laboratory, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Department of Infectious Disease, Shanghai Key Laboratory of Infectious Diseases and Biosafety Emergency Response, National Medical Center for Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China.
Department of Laboratory Medicine, Shanghai Public Health Clinical Center, Shanghai, China.

Zhangyufan He (Z)

Department of Infectious Disease, Shanghai Key Laboratory of Infectious Diseases and Biosafety Emergency Response, National Medical Center for Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China.

Shaoshuai Li (S)

Department of Laboratory Medicine, Shanghai Public Health Clinical Center, Shanghai, China.
Department of Immunology, School of Basic Medical, Jiamusi University, Jiamusi, China.

Dongmei Yan (D)

Department of Immunology, School of Basic Medical, Jiamusi University, Jiamusi, China.

Zhaoqin Zhu (Z)

Department of Laboratory Medicine, Shanghai Public Health Clinical Center, Shanghai, China.

Yanmin Wan (Y)

Department of Infectious Disease, Shanghai Key Laboratory of Infectious Diseases and Biosafety Emergency Response, National Medical Center for Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China.
State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai, China.
Department of Radiology, Shanghai Public Health Clinical Center, Shanghai, China.

Wanhai Wang (W)

Department of Medical Laboratory, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

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Classifications MeSH